Visual morphometry and three non-invasive markers in the evaluation of liver fibrosis in chronic liver disease

Figures & data

Table 1. Baseline characteristics of study population.

Parameter	N = 115
Age (years)	49.7 ± 11.7
Gender
Male	89 (77.4%)
Female	26 (22.6%)
Etiology
Alcohol related liver disease	15 (13%)
Hemochromatosis	3 (2.6%)
Chronic viral hepatitis	24 (20.9%)
Non-alcoholic fatty liver disease	64 (55.7%)
Normal	6 (5.2%)
Others	3 (2.6%)
Biopsy length (mm)	29.6 ± 6.9
Biopsy width (mm)	0.98 ± 0.17
Biopsy area (mm^2)	29.6 ± 10.2
Fibrosis stage
0	24 (20.9%)
1	32 (27.8%)
2	25 (21.7%)
3	22 (19.1%)
4	12 (10.4%)
Pathologists visual morphometric estimate
Mean fibrosis (%)	7.1 ± 7.7

Figure 1. Box plots of the distributions of (A) pathologists estimate of fibrosis, (B) LSM, (C) ELF score and (D) MRI T₁ relaxation time with fibrosis stage.

Table 2. Study parameters distribution by fibrosis stage.

	No cirrhosis	Cirrhosis	p Value	Non-advancedfibrosis	Advancedfibrosis	p Value	F0	F1	F2	F3	F4	p Value(ANOVA)
Fibrosis (%)	5.2 ± 4.0	23.5 ± 11.4	.000	3.7 ± 2.2	15.3 ± 9.6	.000	1.5 ± 0.6	3.7 ± 1.5	5.8 ± 1.8	10.8 ± 4.2	23.5 ± 11.4	.000
LSM (kPa)	7.4 ± 4.5	40.9 ± 24.4	.000	6.0 ± 2.3	19.5 ± 18.4	.000	5.0 ± 1.9	5.3 ± 1.5	7.7 ± 2.6	11.7 ± 6.7	40.9 ± 24.4	.000
ELF score	9.0 ± 1.2	10.9 ± 0.9	.000	8.8 ± 1.1	10.1 ± 1.4	.000	8.5 ± 1.0	8.6 ± 1.2	9.2 ± 1.0	9.8 ± 1.5	10.9 ± 0.9	.000
T1 relaxation time (ms)	651 ± 51	752 ± 79	.002	640 ± 39	711 ± 78	.000	632 ± 37	642 ± 36	646 ± 45	690 ± 70	752 ± 79	.000

ELF: enhanced liver fibrosis; LSM: liver stiffness measurement.

Figure 2. Area under receiver operating curves (AUROC) of study variables for advanced fibrosis.

Figure 3. Area under receiver operating curves (AUROC) of study variables for cirrhosis.

Figure 4. Scatter plot of distribution of (A) LSM, (B) ELF score and (C) T₁ relaxation time with pathologist’s estimate of fibrosis in F3–F4 group.

Figure 5. Scatter plot of distribution of (A) LSM, (B) ELF score and (C) T₁ relaxation time with pathologist’s estimate of fibrosis in F0–F2 group.

Table 3. Correlation of study parameters with pathologist’s estimate of fibrosis.

	LSM (kPa)	ELF score	T1 relaxation time (ms)	LSM (kPa)	ELF score	T1 relaxation time (ms)
	F0–F2 subgroup			F3–F4 subgroup
Spearman’s rho	0.536	0.329	0.111	0.757	0.721	0.723
p Value	.001	.03	.325	.001	<.001	<.001