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Plant nutrition

Selective induction of putative iron transporters, OPT8a and OPT8b, in maize by mycorrhizal colonization

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Pages 843-847 | Received 29 Apr 2014, Accepted 26 Jul 2014, Published online: 20 Aug 2014

Figures & data

Figure 1 Iron-59 accumulation in the shoots of inoculated or non-inoculated Zea mays L. in two-compartment cultivation system. Four maize plants for each treatment (inoculated, Myc+; non-inoculated, Myc–) were grown in two-compartment cultivation system. The shoots were harvested and analyzed at 44 days post planting (dpp). Iron-59 (III) chloride was injected into hyphal compartment at 14 dpp. Data are given as means ± standard deviation (SD) of four biological replicates. N.D., not detected.

Figure 1 Iron-59 accumulation in the shoots of inoculated or non-inoculated Zea mays L. in two-compartment cultivation system. Four maize plants for each treatment (inoculated, Myc+; non-inoculated, Myc–) were grown in two-compartment cultivation system. The shoots were harvested and analyzed at 44 days post planting (dpp). Iron-59 (III) chloride was injected into hyphal compartment at 14 dpp. Data are given as means ± standard deviation (SD) of four biological replicates. N.D., not detected.

Figure 2 The expression analysis of genes related to Iron uptake in mycorrhizal or non-mycorrhizal roots of maize. Real-time RT-PCR-based expression analysis of maize genes related to Iron uptake system in the mycorrhizal (Myc+, R. irregularis) or non-mycorrhizal (Myc–) roots of Zea mays L. Root samples were harvested at 20 days post planting (dpp). Expression levels are shown relative to the constitutively expressed maize ubiquitin gene. Data are given as means ± standard deviation (SD) of three biological replicates. ** P < 0.01, * P < 0.05, Welch’s t test (Myc+ versus Myc–treatment).

Figure 2 The expression analysis of genes related to Iron uptake in mycorrhizal or non-mycorrhizal roots of maize. Real-time RT-PCR-based expression analysis of maize genes related to Iron uptake system in the mycorrhizal (Myc+, R. irregularis) or non-mycorrhizal (Myc–) roots of Zea mays L. Root samples were harvested at 20 days post planting (dpp). Expression levels are shown relative to the constitutively expressed maize ubiquitin gene. Data are given as means ± standard deviation (SD) of three biological replicates. ** P < 0.01, * P < 0.05, Welch’s t test (Myc+ versus Myc–treatment).
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