Impact of soil solarization on the ciliate community structure of a greenhouse soil

Figures & data

Figure 1 Soil temperature of solarized (Solar [+]) and non-solarized (Solar [–]) soil plots fertilized with commercial organic fertilizer or liquid fertilizer from shochu waste during the solarization period.

Figure 2 Impact of solarization on the denaturing gradient gel electrophoresis (DGGE) banding patterns of the ciliate community in soil fertilized with (A) commercial organic fertilizer and (B) liquid fertilizer from shochu waste. +, with solarization; –, without solarisation.

Figure 3 Non-metric multi-dimensional scaling using the Bray–Curtis similarity of the denaturing gradient gel electrophoresis (DGGE) banding patterns of polymerase chain reaction (PCR)-amplified ciliate 18S rRNA genes of soil fertilized with (A) commercial organic fertilizer and (B) liquid fertilizer from shochu waste. +, with solarization; –, without solarization.

Figure 4 Impact of solarization on the Bray–Curtis similarity of the denaturing gradient gel electrophoresis (DGGE) patterns among duplicate plots. Soil fertilized with (A) commercial organic fertilizer and (B) liquid fertilizer from shochu waste. Bars represent the standard error of four combinations (duplicate plots × duplicate analyses).

Table 1 Similarities of sequences from the excised bands to sequences in the NCBI database

		Closest relative
Band	Seq.bp	Microorganism	Accession number	Identity (%)	Phylogenetic group
1	613	Oxytricha sp. 1 MD-2012	JQ723978	99	Spirotrichea; Stichotrichia Sporadotrichida
2	591	Oxytricha sp. 1 MD-2012		99
4	612	Oxytricha sp. 1 MD-2012	JQ723978	99	Spirotrichea; Stichotrichia Sporadotrichida
5	591	Oxytricha sp. 1 MD-2012		99
6	591	Oxytricha sp. 1 MD-2012		99
7	588	Cyrtolophosis minor	JQ723962	96	Colpodea; Cyrtolophosidida
8	588	Cyrtolophosis minor		96
9	588	Cyrtolophosis minor		96
10	566	Cyrtolophosis minor		96
11	587	Cyrtolophosis minor	JQ723962	94	Colpodea; Cyrtolophosidida
12	625	Clone Amb_18S_6341	EF024087	83	Uncultured eukaryote
13	567	Cyrtolophosis minor	JQ723962	95	Colpodea; Cyrtolophosidida
14	572	Metopus laminarius	KF607088	97	Armophorea; ArmophoridaMetopidae