Effects of co-inoculation of Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus P4 on Rj₄ soybean varieties

Figures & data

Table 1. Effect of single or dual inoculation of B. elkanii BLY3-8 strain and B. diazoefficiens USDA110 on nodule number (NN), nodule dry weight (NDW), shoot dry weight (SDW), root dry weight (RDW), and the acetylene reduction activity (ARA) of Yezin-3, Yezin-6, Fukuyutaka, and Akisirome soybean varieties at 30 days after sowing.

Variety	Treatment	NN (number of plant^−1)	NDW (mg plant^−1)	RDW (g plant^−1)	SDW (g plant^−1)	ARA (µmol C2H4 h^−1 plant^−1)
Yezin-3	Control	-	-	0.33 ± 0.04 a	0.61 ± 0.03 b	-
	P4	-	-	0.33 ± 0.01 a	0.64 ± 0.02 b	-
	BLY3-8	16.7 ± 5.7 b	46.54 ± 3.60 b	0.30 ± 0.01 a	0.99 ± 0.12 a	0.83 ± 0.01 b
	BLY3-8 + P4	35.7 ± 7.8 a	59.07 ± 5.08 a	0.35 ± 0.04 a	0.92 ± 0.06 a	1.04 ± 0.04 a
	USDA110	38.7 ± 5.7 a	62.37 ± 2.98 a	0.34 ± 0.03 a	0.93 ± 0.04 a	0.98 ± 0.03 ab
	USDA110 + P4	50.0 ± 2.2 a	66.93 ± 2.17 a	0.38 ± 0.05 a	1.06 ± 0.03 a	1.09 ± 0.05 a
Yezin-6	Control	-	-	0.42 ± 0.03 a	0.69 ± 0.05 b	-
	P4	-	-	0.47 ± 0.04 a	0.92 ± 0.03 a	-
	BLY3-8	12.3 ± 1.9 a	51.23 ± 4.41 a	0.51 ± 0.07 a	1.11 ± 0.09 a	1.48 ± 0.01 a
	BLY3-8 + P4	18.7 ± 4.2 a	54.40 ± 3.06 a	0.44 ± 0.02 a	1.04 ± 0.04 a	1.66 ± 0.03 a
	USDA110	16.0 ± 4.5 a	62.97 ± 9.86 a	0.44 ± 0.06 a	0.93 ± 0.10 a	1.55 ± 0.14 a
	USDA110 + P4	16.3 ± 1.2 a	51.37 ± 5.55 a	0.43 ± 0.06 a	0.99 ± 0.02 a	1.55 ± 0.26 a
Fukuyutaka	Control	-	-	0.46 ± 0.06 a	0.82 ± 0.04 a	-
	P4	-	-	0.54 ± 0.06 a	0.99 ± 0.02 a	-
	BLY3-8	10.0 ± 0.8 c	22.27 ± 0.76 c	0.51 ± 0.04 a	0.97 ± 0.03 a	0.64 ± 0.04 c
	BLY3-8 + P4	21.3 ± 2.4 b	35.07 ± 0.86 b	0.51 ± 0.07 a	0.92 ± 0.04 a	1.03 ± 0.16 a
	USDA110	28.3 ± 1.2 ab	51.13 ± 3.94 a	0.42 ± 0.05 a	0.94 ± 0.09 a	0.71 ± 0.06 bc
	USDA110 + P4	30.3 ± 4.2 a	57.16 ± 6.39 a	0.45 ± 0.02 a	1.04 ± 0.12 a	0.95 ± 0.04 ab
Akisirome	Control	-	-	0.46 ± 0.04 a	0.84 ± 0.01 b	-
	P4	-	-	0.51 ± 0.05 a	0.95 ± 0.01 b	-
	BLY3-8	18.0 ± 3.7 a	38.87 ± 1.74 a	0.50 ± 0.03 a	1.21 ± 0.07 a	1.02 ± 0.04 a
	BLY3-8 + P4	17.7 ± 1.9 a	41.23 ± 4.67 a	0.44 ± 0.02 a	1.20 ± 0.08 a	0.77 ± 0.02 a
	USDA110	19.0 ± 9.5 a	55.03 ± 1.18 a	0.47 ± 0.02 a	1.28 ± 0.08 a	0.78 ± 0.03 a
	USDA110 + P4	20.0 ± 7.3 a	53.73 ± 0.62 a	0.46 ± 0.06 a	1.26 ± 0.05 a	0.93 ± 0.06 a

Mean values ± standard deviation (SD) in each column at each growth stage followed by the same letters are not significantly different at P < 0.05 (Tukey’s test).

Table 2. Effect of co-inoculation of B. elkanii BLY3-8 and S. griseoflavus P4 nodule number (NN), nodule dry weight (NDW), shoot dry weight (SDW), root dry weight (RDW), and shoot N uptake of Yezin-3 soybean variety at different growth stages.

Growth stage	Treatment	NN (number of plant^−1)	NDW (mg plant^−1)	RDW (g plant^−1)	SDW (g plant^−1)	N uptake (mg plant^−1)
V6	Control	10.7 ± 1.7 a	22.00 ± 2.29 a	0.40 ± 0.08 a	1.75 ± 0.34 a	18.34 ± 1.39 a
	P4	8.3 ± 1.7 a	25.27 ± 3.41 a	0.59 ± 0.04 a	2.33 ± 0.13 a	23.33 ± 2.36 a
	BLY3-8	10.0 ± 2.8 a	33.30 ± 5.91 a	0.65 ± 0.12 a	2.53 ± 0.45 a	22.70 ± 3.60 a
	BLY3-8 + P4	7.7 ± 1.7 a	27.73 ± 4.53 a	0.49 ± 0.13 a	2.13 ± 0.32 a	18.15 ± 3.90 a
R3.5	Control	56.0 ± 11.6 b	403.33 ± 18.86 b	1.31 ± 0.05 b	7.34 ± 0.20 b	161.92 ± 2.92 b
	P4	73.3 ± 3.4 ab	460.00 ± 14.14 ab	1.59 ± 0.05 ab	7.87 ± 0.20 ab	173.04 ± 7.75 ab
	BLY3-8	78.0 ± 1.6 ab	500.00 ± 35.59 ab	1.60 ± 0.07 ab	8.08 ± 0.21 ab	171.86 ± 5.75 ab
	BLY3-8 + P4	89.3 ± 7.4 a	526.67 ± 47.14 a	1.78 ± 0.16 a	8.40 ± 0.33 a	187.98 ± 4.51 a
R8	Control	-	-	-	9.21 ± 0.51 b	158.98 ± 8.87 b
	P4	-	-	-	11.25 ± 0.44 a	187.94 ± 7.46 ab
	BLY3-8	-	-	-	11.34 ± 0.83 a	205.29 ± 27.69 ab
	BLY3-8 + P4	-	-	-	11.51 ± 0.52 a	227.95 ± 10.79 a

Mean values ± standard deviation (SD) in each column at each growth stage followed by the same letters are not significantly different at P < 0.05 (Tukey’s test).

Table 3. Effect of co-inoculation of B. elkanii BLY3-8 and S. griseoflavus P4 nodule number (NN), nodule dry weight (NDW), shoot dry weight (SDW), root dry weight (RDW), and shoot N uptake of Fukuyutaka soybean variety at different growth stages.

Growth stage	Treatment	NN (number of plant^−1)	NDW (mg plant^−1)	RDW (g plant^−1)	SDW (g plant^−1)	N uptake (mg plant^−1)
V6	Control	10.0 ± 0.8 b	7.05 ± 0.07 b	0.65 ± 0.08 a	2.07 ± 0.30 a	24.47 ± 0.59 a
	P4	14.0 ± 1.4 ab	6.01 ± 1.40 b	0.69 ± 0.12 a	2.18 ± 0.50 a	26.20 ± 2.32 a
	BLY3-8	20.3 ± 0.5 ab	9.40 ± 5.07 b	0.78 ± 0.12 a	2.77 ± 0.40 a	32.71 ± 9.37 a
	BLY3-8 + P4	21.0 ± 5.0 a	19.23 ± 0.99 a	0.79 ± 0.01 a	2.74 ± 0.02 a	32.05 ± 4.29 a
R3.5	Control	101.7 ± 8.2 b	480.00 ± 49.67 b	4.07 ± 0.01 b	8.35 ± 0.14 b	179.31 ± 35.34 b
	P4	120.0 ± 4.1 ab	580.00 ± 8.16 ab	4.65 ± 0.13 ab	8.88 ± 0.40 ab	204.44 ± 3.30 ab
	BLY3-8	122.7 ± 7.7 ab	566.67 ± 47.14 ab	5.03 ± 0.41 ab	9.68 ± 0.85 ab	230.90 ± 17.23 ab
	BLY3-8 + P4	126.7 ± 3.4 a	653.33 ± 12.47 a	5.54 ± 0.42 a	10.69 ± 0.80 a	248.07 ± 15.22 a
R8	Control	-	-	-	10.46 ± 0.57 b	225.64 ± 8.25 b
	P4	-	-	-	12.37 ± 0.34 a	284.40 ± 20.78 a
	BLY3-8	-	-	-	12.36 ± 0.24 a	271.38 ± 9.84 a
	BLY3-8 + P4	-	-	-	12.38 ± 0.33 a	280.25 ± 7.34 a

Mean values ± standard deviation (SD) in each column at each growth stage followed by the same letters are not significantly different at P < 0.05 (Tukey’s test).

Figure 1. Effect of co-inoculation of B. elkanii BLY3-8 and S. griseoflavus on (A) acetylene reduction activity (ARA) at V6 stage, (B) relative ureide index (%) at R3.5 stage, (C) N derived from N fixation (%Ndfa) at R3.5 of Yezin-3 and Fukuyutaka soybean varieties. The histograms with the same letter at each variety are not significantly different at P < 0.05 (Tukey’s test). The bar on each histogram indicates standard deviation (SD).

Table 4. Effect of co-inoculation of B. elkanii BLY3-8 and S. griseoflavus on seed yield and yield components of Yezin-3 and Fukuyutaka soybean varieties at maturity stage.

Variety	Treatment	Pods (number of plant^−1)	Seeds per pod (number of pod^−1)	100 seed weight (g)	Seed yield (g plant^−1)
Yezin-3	Control	17.7 ± 1.7 a	2.0 ± 0.2 a	13.62 ± 0.68 a	4.63 ± 0.32 b
	P4	24.7 ± 4.7 a	1.7 ± 0.2 a	13.19 ± 0.85 a	5.29 ± 0.25 ab
	BLY3-8	24.3 ± 1.2 a	1.9 ± 0.1 a	11.82 ± 1.33 a	5.41 ± 0.43 ab
	BLY3-8 + P4	20.0 ± 2.2 a	2.2 ± 0.2 a	13.85 ± 0.58 a	6.04 ± 0.14 a
Fukuyutaka	Control	13.3 ± 0.5 a	1.7 ± 0.1 a	24.17 ± 1.20 a	5.46 ± 0.17 b
	P4	17.3 ± 0.9 a	1.6 ± 0.2 a	22.86 ± 0.99 a	6.32 ± 0.09 a
	BLY3-8	16.7 ± 0.5 a	1.7 ± 0.1a	23.04 ± 2.34 a	6.40 ± 0.19 a
	BLY3-8 + P4	16.3 ± 0.9 a	1.8 ± 0.1 a	23.53 ± 0.31 a	6.74 ± 0.20 a

Mean values ± standard deviation (SD) in each column followed by the same letters are not significantly different at P < 0.05 (Tukey’s test).