Selection of type A and type B strains for improving symbiotic effectiveness on non-Rj and Rj₄ genotype soybean varieties

Figures & data

Table 1. Effect of inoculation of selected Bradyrhizobium type A strains on NN, NDW, RDW and SDW of Yezin-3 and Yezin-6 soybean varieties at 30 days after sowing.

Cultivar	Strain	NN (No. plant^−1)	NDW (mg plant^−1)	RDW (g plant^−1)	SDW (g plant^−1)
Yezin-3	AHY3-1	20.3 ± 3.1 a	29.57 ± 1.42 a	0.23 ± 0.04 a	0.58 ± 0.04 a
	AHY3-6	11.7 ± 1.9 bc	21.80 ± 0.66 a	0.25 ± 0.05 a	0.52 ± 0.09 a
	AHY6-1	12.7 ± 1.2 bc	23.63 ± 2.98 a	0.22 ± 0.03 a	0.52 ± 0.08 a
	BLY6-5	18.3 ± 2.4 ab	32.60 ± 3.40 a	0.31 ± 0.03 a	0.55 ± 0.02 a
	MMY3-1	10.7 ± 0.9 c	29.83 ± 2.78 a	0.28 ± 0.03 a	0.54 ± 0.04 a
	MMY3-2	13.3 ± 1.2 bc	27.73 ± 3.21 a	0.27 ± 0.03 a	0.51 ± 0.04 a
	SAY3-7	12.3 ± 0.5 bc	31.23 ± 2.86 a	0.22 ± 0.04 a	0.55 ± 0.04 a
	SAY6-2	20.7 ± 3.7 a	30.70 ± 4.48 a	0.21 ± 0.02 a	0.49 ± 0.02 a
	SMY3-5	9.7 ± 0.5 c	33.23 ± 4.87 a	0.23 ± 0.01 a	0.50 ± 0.03 a
	SMY6-1	12.0 ± 1.4 bc	27.37 ± 3.55 a	0.23 ± 0.02 a	0.48 ± 0.02 a
	USDA110	13.3 ± 0.9 bc	25.37 ± 4.32 a	0.22 ± 0.03 a	0.51 ± 0.04 a
Yezin-6	AHY3-1	20.0 ± 1.6 ab	63.13 ± 3.31 a	0.43 ± 0.08 a	0.72 ± 0.06 a
	AHY3-6	10.0 ± 1.2 c	33.67 ± 5.68 cd	0.41 ± 0.06 a	0.61 ± 0.07 a
	AHY6-1	15.0 ± 0.8 bc	38.90 ± 0.67 bcd	0.34 ± 0.02 a	0.54 ± 0.09 a
	BLY6-5	15.3 ± 1.2 bc	40.63 ± 3.84 bcd	0.42 ± 0.04 a	0.58 ± 0.02 a
	MMY3-1	11.7 ± 1.9 c	30.37 ± 4.91 cd	0.39 ± 0.04 a	0.63 ± 0.00 a
	MMY3-2	11.7 ± 2.4 c	36.77 ± 2.42 cd	0.34 ± 0.01 a	0.66 ± 0.02 a
	SAY3-7	11.7 ± 2.4 c	63.97 ± 4.68 a	0.43 ± 0.06 a	0.65 ± 0.05 a
	SAY6-2	24.0 ± 2.9 a	52.57 ± 1.93 ab	0.45 ± 0.08 a	0.60 ± 0.06 a
	SMY3-5	19.0 ± 2.2 ab	61.07 ± 5.59 a	0.39 ± 0.02 a	0.60 ± 0.06 a
	SMY6-1	11.7 ± 1.9 c	28.6 ± 3.82 d	0.35 ± 0.03 a	0.61 ± 0.05 a
	USDA110	21.0 ± 2.2 ab	46.43 ± 2.85 bc	0.41 ± 0.02 a	0.71 ± 0.02 a

Mean value ± SD in each column at each variety followed by the same letter is not significantly different at P < 0.05 (Tukey’s test).

Figure 1. Effect of inoculation of Bradyrhizobium type A strains on acetylene reduction activity (ARA) of Yezin-6 in the first screening (A), and Yezin-3 and Yezin-6 in the second screening (B) at 30 days after sowing. The histograms with the same letter at each variety are not significantly different at P < 0.05 (Tukey’s test). The bar on each histogram indicates standard deviation (SD).

Figure 2. Effect of inoculation of Bradyrhizobium type B strains on ARA of Yezin-3 in the first screening (A), and Yezin-3 and Yezin-6 in the second screening (B) at 30 days after sowing. The histograms with the same letter at each variety are not significantly different at P < 0.05 (Tukey’s test). The bar on each histogram indicates SD.

Table 2. Effect of inoculation of selected Bradyrhizobium type B strains on NN, NDW, RDW, and SDW of Yezin-3 and Yezin-6 soybean varieties at 30 days after sowing.

Cultivar	Strain	NN (No. plant^−1)	NDW (mg plant^−1)	RDW (g plant^−1)	SDW (g plant^−1)
Yezin-3	BLY3-8	27.0 ± 1.6 a	53.50 ± 0.85 a	0.32 ± 0.02 a	1.17 ± 0.08 a
	BLY6-1	16.3 ± 0.5 bc	39.03 ± 3.97 c	0.36 ± 0.02 a	1.25 ± 0.03 a
	SAY3-3	15.3 ± 1.7 c	38.90 ± 3.65 c	0.33 ± 0.04 a	1.15 ± 0.11 a
	SAY3-10	24.7 ± 1.2 a	54.73 ± 1.39 a	0.32 ± 0.04 a	1.07 ± 0.12 a
	SHY3-1	24.7 ± 1.7 a	56.23 ± 3.49 a	0.32 ± 0.07 a	0.94 ± 0.14 a
	SHY3-3	18.7 ± 2.6 bc	44.00 ± 1.50 bc	0.27 ± 0.01 a	1.00 ± 0.15 a
	SHY6-1	18.3 ± 0.9 bc	41.13 ± 0.24 c	0.32 ± 0.01 a	0.98 ± 0.07 a
	SHY6-5	10.0 ± 0.8 c	45.07 ± 2.60 bc	0.34 ± 0.02 a	0.95 ± 0.10 a
	SHY6-6	18.7 ± 0.5 bc	42.57 ± 1.79 bc	0.37 ± 0.04 a	1.07 ± 0.08 a
	SHY6-10	15.0 ± 0.8 c	45.07 ± 0.73 bc	0.28 ± 0.02 a	0.90 ± 0.08 a
	USDA110	21.7 ± 2.4 ab	50.67 ± 1.59 ab	0.26 ± 0.04 a	0.91 ± 0.11 a
Yezin-6	BLY3-8	33.0 ± 2.2 a	75.80 ± 1.14 a	0.40 ± 0.05 a	1.37 ± 0.18 a
	BLY6-1	14.7 ± 3.7 d	45.73 ± 2.29 c	0.40 ± 0.04 a	1.07 ± 0.08 a
	SAY3-3	25.0 ± 0.8 bc	56.80 ± 4.24 b	0.46 ± 0.05 a	1.18 ± 0.16 a
	SAY3-10	32.7 ± 1.9 ab	73.67 ± 0.45 a	0.45 ± 0.05 a	1.11 ± 0.08 a
	SHY3-1	33.0 ± 2.2 a	74.37 ± 0.69 a	0.33 ± 0.04 a	1.05 ± 0.03 a
	SHY3-3	22.0 ± 2.2 cd	62.50 ± 2.55 b	0.44 ± 0.12 a	1.17 ± 0.13 a
	SHY6-1	14.7 ± 2.9 d	60.13 ± 2.87 b	0.39 ± 0.09 a	1.17 ± 0.17 a
	SHY6-5	25.0 ± 0.8 bc	62.47 ± 3.73 b	0.30 ± 0.03 a	0.94 ± 0.02 a
	SHY6-6	22.0 ± 2.2 cd	59.03 ± 1.60 b	0.43 ± 0.04 a	1.17 ± 0.05 a
	SHY6-10	17.0 ± 1.6 d	63.10 ± 4.82 b	0.36 ± 0.03 a	1.02 ± 0.08 a
	USDA110	35.3 ± 2.5 a	75.07 ± 1.33 a	0.36 ± 0.07 a	1.04 ± 0.09 a

Mean value ± SD in each column at each variety followed by the same letter is not significantly different at P < 0.05 (Tukey’s test).

Table 3. Effect of inoculation of selected B. elkanii AHY3-1 (type A), B. elkanii BLY3-8 (type B), B. japnicum SAY3-7 (type A) and B. japnicum SAY3-10 (type B) strains on NN, NDW, RDW, and SDW of Yezin-3, Yezin-6, Yezin-11 and Yezin-14 soybean varieties at 30 days after sowing.

Variety	Strain	NN (No. plant^−1)	NDW (mg plant^−1)	RDW (g plant^−1)	SDW (g plant^−1)
Yezin-3	AHY3-1	18.0 ± 1.4 ab	39.70 ± 0.50 b	0.41 ± 0.03 a	0.89 ± 0.12 a
	BLY3-8	23.0 ± 2.5 a	55.50 ± 4.70 a	0.40 ± 0.07 a	0.96 ± 0.06 a
	SAY3-7	19.7 ± 1.3 ab	49.50 ± 1.02 a	0.32 ± 0.02 a	0.91 ± 0.04 a
	SAY3-10	12.0 ± 1.6 c	34.83 ± 2.65 b	0.37 ± 0.09 a	1.10 ± 0.30 a
	USDA110	15.0 ± 1.6 bc	36.87 ± 0.90 b	0.38 ± 0.02 a	0.87 ± 0.10 a
Yezin-6	AHY3-1	17.3 ± 3.3 a	45.07 ± 1.16 b	0.43 ± 0.03 a	0.98 ± 0.03 a
	BLY3-8	14.3 ± 1.3 a	46.23 ± 6.06 b	0.42 ± 0.01 a	0.97 ± 0.02 a
	SAY3-7	14.3 ± 1.3 a	70.37 ± 3.03 a	0.48 ± 0.08 a	1.03 ± 0.04 a
	SAY3-10	17.3 ± 0.5 a	56.20 ± 2.76 b	0.43 ± 0.03 a	1.08 ± 0.06 a
	USDA110	19.0 ± 0.8 a	45.57 ± 3.74 b	0.46 ± 0.01 a	0.97 ± 0.13 a
Yezin-11	AHY3-1	19.0 ± 2.8 a	33.53 ± 4.52 a	0.46 ± 0.07 a	0.88 ± 0.03 a
	BLY3-8	18.7 ± 0.5 a	32.80 ± 2.61 a	0.44 ± 0.06 a	0.91 ± 0.03 a
	SAY3-7	15.3 ± 2.5 a	37.00 ± 4.24 a	0.38 ± 0.06 a	0.88 ± 0.01 a
	SAY3-10	16.0 ± 1.4 a	40.00 ± 1.50 a	0.55 ± 0.06 a	1.00 ± 0.07 a
	USDA110	18.3 ± 2.1 a	33.13 ± 0.92 a	0.42 ± 0.05 a	0.89 ± 0.04 a
Yezin-14	AHY3-1	19.0 ± 1.6 a	41.70 ± 3.10 c	0.38 ± 0.05 a	0.97 ± 0.05 a
	BLY3-8	15.3 ± 3.9 a	57.10 ± 4.29 ab	0.51 ± 0.01 a	1.21 ± 0.05 a
	SAY3-7	18.7 ± 2.5 a	61.60 ± 8.42 a	0.42 ± 0.05 a	1.03 ± 0.17 a
	SAY3-10	22.7 ± 4.2 a	45.50 ± 0.82 bc	0.42 ± 0.09 a	1.10 ± 0.14 a
	USDA110	20.3 ± 0.9 a	43.73 ± 2.80 bc	0.39 ± 0.03 a	1.01 ± 0.15 a

Mean value ± SD in each column at each variety followed by the same letter is not significantly different at P < 0.05 (Tukey’s test).

Figure 3. Effect of inoculation of selected B. elkanii AHY3-1 (type A), B. elkanii BLY3-8 (type B), B. japnicum SAY3-7 (type A) and B. japnicum SAY3-10 (type B) strains on ARA of Yezin-3, Yezin-6, Yezin-11 and Yezin-14 soybean varieties at 30 days after sowing. The histograms with the same letter at each variety are not significantly different at P < 0.05 (Tukey’s test). The bar on each histogram indicates SD.

Table 4. Effect of inoculation of B. japonicum SAY3-7 (type A) and B. elkanii BLY3-8 (type B) on NN, NDW, RDW, and SDW of Yezin-3 and Yezin-6 soybean varieties at different growth stages.

Growth Stage	Variety	Treatment	NN (No. plant^−1)	NDW (mg plant^−1)	RDW (g plant^−1)	SDW (g plant^−1)
V6	Yezin-3	Control	9.7 ± 0.5 a	24.70 ± 0.71 a	0.49 ± 0.11 a	2.01 ± 0.31 a
		SAY3-7	11.3 ± 1.9 a	31.0 ± 0.73 a	0.51 ± 0.04 a	1.91 ± 0.05 a
		BLY3-8	11.3 ± 1.9 a	27.73 ± 4.53 a	0.49 ± 0.13 a	2.13 ± 0.32 a
	Yezin-6	Control	10.3 ± 0.5 a	22.40 ± 0.96 b	0.41 ± 0.01 b	1.51 ± 0.05 b
		SAY3-7	14.0 ± 1.4 a	34.83 ± 2.22 a	0.51 ± 0.04 a	1.68 ± 0.03 a
		BLY3-8	12.7 ± 1.7 a	30.63 ± 0.75 a	0.53 ± 0.02 a	1.73 ± 0.00 a
R3.5	Yezin-3	Control	55.3 ± 10.7 b	413.33 ± 17.00 b	1.38 ± 0.11 a	7.38 ± 0.15 b
		SAY3-7	63.3 ± 2.4 ab	476.67 ± 20.55 ab	1.69 ± 0.24 a	7.75 ± 0.07 ab
		BLY3-8	86.0 ± 8.5 a	523.33 ± 44.97 a	1.64 ± 0.06 a	8.38 ± 0.34 a
	Yezin-6	Control	72.7 ± 5.3 b	376.67 ± 4.71 b	1.85 ± 0.04 b	7.22 ± 0.14 b
		SAY3-7	93.7 ± 4.5 a	456.67 ± 17.00 a	2.07 ± 0.06 a	7.80 ± 0.03 a
		BLY3-8	92.0 ± 2.2 a	473.33 ± 17.00 a	2.07 ± 0.06 a	7.80 ± 0.03a
R8	Yezin-3	Control	–	–	–	9.21 ± 0.51 b
		SAY3-7	–	–	–	11.45 ± 0.40 a
		BLY3-8	–	–	–	11.51 ± 0.52 a
	Yezin-6	Control	–	–	–	11.0 ± 0.84 b
		SAY3-7	–	–	–	13.37 ± 0.41 a
		BLY3-8	–	–	–	13.31 ± 0.45 a

Mean value ± SD in each column at each growth stage at each variety followed by the same letter is not significantly different at P < 0.05 (Tukey’s test). Control means un-inoculated treatment.

Figure 4. Effect of inoculation of B. japonicum SAY3-7 (type A) and B. elkanii BLY3-8 (type B) on (A) ARA at V6 stage, (B) relative ureide index (%) at R3.5 stage, (C) N derived from N fixation (%Ndfa) at R3.5 of Yezin-3 and Yezin-6 soybean varieties. The histograms with the same letter at each variety are not significantly different at P < 0.05 (Tukey’s test). The bar on each histogram indicates SD.

Table 5. Effect of inoculation of B. japonicum SAY3-7 (type A) and B. elkanii BLY3-8 (type B) on yield components and seed yield of Yezin-3 and Yezin-6 soybean varieties at maturity stage.

Variety	Treatment	Pods (No. plant^−1)	Seeds per pod (No. pod^−1)	100 seed weight (g)	Seed yield (g plant^−1)
Yezin-3	Control	18.0 ± 1.6 a	1.97 ± 0.2 a	14.08 ± 0.24 a	4.92 ± 0.14 b
	SAY3-7	19.0 ± 1.4 a	1.88 ± 0.2 a	14.08 ± 0.31 a	5.65 ± 0.16 a
	BLY3-8	20.0 ± 2.2 a	2.20 ± 0.2 a	13.70 ± 0.55 a	5.86 ± 0.24 a
Yezin-6	Control	18.7 ± 1.9 a	2.01 ± 0.1 a	14.31 ± 0.93 a	5.31 ± 0.25 b
	SAY3-7	22.0 ± 1.4 a	1.97 ± 0.1 a	14.71 ± 0.80 a	6.36 ± 0.30 a
	BLY3-8	21.0 ± 1.4 a	1.99 ± 0.1 a	15.33 ± 0.17 a	6.38 ± 0.29 a

Mean value ± SD in each column at each variety followed by the same letter is not significantly different at P < 0.05 (Tukey’s test). Control means un-inoculated treatment.