Investigation on the conformational changes of bovine serum albumin in a wide pH range from 2 to 12

Abstract

The conformation of bovine serum albumin largely depends on its microenvironment pH and affects its physical functions and applications. In this study, we investigated the effects of pH (wide range 2–12) on the conformation of bovine serum albumin based on spectroscopic signals by various spectroscopic means including fluorescence, synchronous fluorescence, resonance light scattering, UV-visible absorption, and circular dichroism. The changes in spectroscopic signals, such as peak position and intensity, showed that the structure of bovine serum albumin varied significantly with pH. The conformation of bovine serum albumin was compact at pH 6–7, as indicated by the largest peak position values and peak intensities in the fluorescence, synchronous fluorescence, and RLS spectra. The structure of bovine serum albumin became loose in the acidic or alkaline environment, as indicated by the decreased peak position values and peak intensities. The microenvironment of the amino acid residues of bovine serum albumin also varied with pH, as indicated by the changing peak position values. At pH 7, the hydrophobicity of the tyrosine and tryptophan residues was the weakest, as indicated by the minimum synchronous fluorescence signals. In addition, the secondary structure of bovine serum albumin, especially α-helix, varied with pH. The content of α-helix reached the maximum value of 68% at pH 6–8, whereas it decreased in the acidic or alkaline environment. The study provides valuable details for studying the physiological function and applications of serum albumins.

Keywords:

• Bovine serum albumin
• conformation
• microenvironment
• pH
• spectroscopy

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [21707026], the Guangzhou Key Laboratory of Environmental Exposure and Health [No. GZKLEEH201613], and the Shandong Provincial Natural Science Foundation, China [ZR2014BQ033]. The Natural Scientific Research Innovation Foundation in Harbin Institute of Technology [HIT.NSRIF.2014126] is also acknowledged.