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Australian Journal of Forensic Sciences Volume 48, 2016 - Issue 1
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Improved high throughput processing of touch DNA samples

Ruhua ZhouPublic Security Bureau of Suzhou, Institute of Forensic Science, Suzhou, China
,
Qingzhen MengMinistry of Public Security, Institute of Forensic Science, Beijing, China;Ministry of Public Security, Key Laboratory of Forensic Genetics, Beijing, China
,
Pengfei LiPublic Security Bureau of Suzhou, Institute of Forensic Science, Suzhou, China
,
Jian ChenPublic Security Bureau of Suzhou, Institute of Forensic Science, Suzhou, China
,
Yuanpeng SunPublic Security Bureau of Suzhou, Institute of Forensic Science, Suzhou, China
&
Min LiPublic Security Bureau of Suzhou, Institute of Forensic Science, Suzhou, ChinaCorrespondence[email protected]
Pages 2-9 | Received 05 Nov 2014, Accepted 03 Feb 2015, Published online: 13 Apr 2015
 
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Abstract

We report the design of a 96-well centrifugal filtration plate and its use in an automated DNA extraction method of touched objects. Cells on the touched objects such as glove prints, swabs from door handles, tools, and so on, are lysed in the 96-well filtration plate. The cell lysate is separated from the objects by centrifugation. Then automated DNA extraction and purification are carried out automatically on a robotic workstation. Such a method not only improves the DNA profile obtained from touched objects but also shortens sample processing time. Processing of 92 samples can be completed in 90 min.

Competing interests

The authors declare no conflict of interest.

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