Effect of a one-off sporidesmin challenge on the milk production of dairy cows

Abstract

AIMS

To investigate the effects on milk yield in lactating dairy cows of a single dose of sporidesmin, and to categorise the responses based on clinical signs and differing degrees of liver damage, as assessed by activities of γ-glutamyl transferase (GGT) and post-mortem liver histopathology.

METHODS

Adult lactating dairy cows (n=17) were given a single intra-ruminal dose of 0.24 mg/kg of sporidesmin dissolved in ethanol and diluted in water on Day 0; an additional three cows served as untreated controls. Weekly serum samples were collected between Days −14 and 42 and analysed for activities of GGT. Milk yields were measured daily over the same period. Cows were subjected to euthanasia due to severe clinical signs (n=2) or were slaughtered at the end of the trial. Samples of livers were examined histologically and were scored for lesions on a scale from 0 (normal) to 3 (severe). Based on GGT activities and clinical observations, cows that were treated with sporidesmin were categorised as non-responders (no clinical signs and normal GGT), subclinical (elevated GGT and no clinical signs) or clinical. Outcomes were compared between these three groups and control cows using generalised additive models.

RESULTS

Seven cows were classified as clinical, and had median liver scores of 22 (95% CI=20.6–23.4), six were subclinical with median liver scores of 8.7 (95% CI=3.8–13.5) and four were non-responders with median liver scores of 2.5 (95% CI=1.2–4.3). Median liver scores for the three control cows were 1 (95% CI=−0.8–2.1). Activities of GGT increased in subclinical and clinical cows around Day 7. The milk yield of all cows treated with sporidesmin, including non-responder cows, started to decrease on Day 1, and reached a nadir (a drop of between 9 and 85%) on Day 7.

CONCLUSIONS AND CLINICAL RELEVANCE

It is likely that the overall effects of sporidesmin consumption on milk production by the national herd in New Zealand are hugely underestimated, especially considering its effects on non-responder and subclinical cows as shown in this trial. In view of the results presented here, the authors are suggesting a change to the definition of response to sporidesmin from non-responder, subclinical, and clinical, to subclinical-low, subclinical-high, and clinical, when measuring a combination of GGT activities, clinical signs and milk yields during facial eczema-risk seasons (summer–autumn).

KEY WORDS:

• Facial eczema
• sporidesmin
• non-responders
• milk production
• dairy cattle

View correction statement:

Re: Effect of a one-off sporidesmin challenge on the milk production of dairy cows

Acknowledgements

Funding for this work was provided by grants from the Ministry of Business, Innovation and Employment, and the Sinclair Cummings Scholarship. We thank the staff at Massey University Dairy No. 4, and those at New Zealand Veterinary Pathology. Special thanks to Aaron Gilmour for his enthusiastic help during the trial, and Neville Amyes for all of his knowledge.