Enzootic calcinosis in Toggenburg goats in New Zealand

Figures & data

Figure 1. Photographs of tissues from a 13-year-old female Toggenburg goat with soft tissue mineralisation showing a) thoracic aorta with raised, white intimal plaques; b) heart with white mineralised plaques on the aorta, left atrioventricular valves and aortic outflow tract; and c) lung with raised, firm, mineralised nodules.

Figure 2. Photomicrographs of tissues from Toggenburg goats with soft tissue mineralisation. a) Mineralisation of small muscular artery in the abomasal submucosa in an 11-year-old female goat (H&E; bar = 100 μm). b) Osteochondroid metaplasia of aorta with osteoid in areas where vessels have infiltrated mineralised foci in a 6-year-old male goat (H&E; bar = 400 μm). c) Extensive basophilic amorphous mineral and early osseous metaplasia expanding alveolar septa of the lung in an 11-year-old female goat (H&E; bar = 400 μm). d) The presence of mineral in alveolar septa is highlighted by Von Kossa stain showing a honeycomb arrangement in a 9-year-old male goat (bar = 200 μm).

Figure 3. Photomicrographs of sections of bone from a lumbar vertebra of a 13-year-old female Toggenburg goat with soft tissue mineralisation showing abnormal basophilic floccular matrix lining a) bone lamellae and b) osteons (H&E; bar 100 μm).

Figure 4. Graph of absolute values for concentrations of (a) 25-hydroxyvitamin D₂ (25OHD₂; nmol/L), (b) 25-hydroxyvitamin D₃ (25OHD₃; nmol/L), (c) 25-hydroxyvitamin total D₂ and D₃ (25OHD total; nmol/L), (d) calcium (Ca; mmol/L), and e) phosphorus (P; mmol/L) in serum between April 2018 and March 2019 for goats from a herd affected by soft tissue mineralisation (n = 15; grey solid line), and an unaffected herd (n = 10; grey dotted line). Overlaid are the results of the linear mixed effects model for affected goats (black solid line) and unaffected goats (black dashed line), with the grey shading indicating the 95% CI. (f) Graph of monthly ultraviolet B (UVB) radiation.