Solvent-Free Fabrication of Tissue Engineering Scaffolds With Immiscible Polymer Blends

Abstract

A completely organic solvent-free fabrication method is developed for tissue engineering scaffolds by gas foaming of immiscible polylactic acid (PLA) and sucrose blends, followed by water leaching. PLA scaffolds with above 90% porosity and 25–200 µm pore size were fabricated. The pore size and porosity was controlled with process parameters including extrusion temperature and foaming process parameters. Dynamic mechanical analysis showed that the extrusion temperature could be used to control the scaffold strength. Both unfoamed and foamed scaffolds were used to culture glioblastoma (GBM) cells M059 K. The results showed that the cells grew better in the foamed PLA scaffolds. The method presented in the paper is versatile and can be used to fabricate tissue engineering scaffolds without any residual organic solvents.

Keywords:

• GBM cells
• immiscible polymer
• pore size
• porosity
• solvent-free fabrication
• tissue engineering scaffolds

Notes

Error was expressed as ± 1 standard deviation.

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