Affinity Based Laccase Immobilization on Modified Magnetic Nanoparticles: Biosensing Platform for the Monitoring of Phenolic Compounds

Abstract

The authors report an electrochemical phenol biosensor based on the immobilization of laccase (Lac) on the surface of copper capped magnetic core–shell (Fe₃O₄–SiO₂) nanoparticles (MNPs). After synthesis, MNP surfaces were functionalized by silanization and then, modified with histidine (His) and copper, respectively. The performance of the MNP-His/Cu/Lac biosensor has been investigated at −0.05 V versus Ag/AgCl. The proposed biosensor allowed to detect catechol and phenol in the range of 0.01–0.4 mM and 0.025–0.2 mM, respectively. Finally, phenol analysis in culture medium of P. putida adapted to phenol was successfully demonstrated with appreciable recovery values.

GRAPHICAL ABSTRACT

Keywords:

• Bioprocess monitoring
• biosensor
• laccase
• magnetic nanoparticles
• phenolic compounds
• surface modification

Acknowledgments

Drs. Demirkol and Timur contributed equally to this article. Dr. Babadostu acknowledges to TUBITAK for the financial support through 2209–Research Project for university students. The authors thank Prof. Figen Zihnioglu for valuable help in HPLC applications.

Notes

CPE = carbon paste electrode; ANNs = artificial neural networks; MNP-His/Cu/Lac = magnetic nanoparticle/histidine/copper/laccase bionanocomposite.

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