DNA cleavage activities of two dinuclear copper coordination polymers

Abstract

The DNA cleavage activities of two coordination polymers of Robson-type macrocycles, {[Cu₄L¹(4,4′-bipy)₂](ClO₄)₄·H₂O}_∞ (1) and {[Cu₄L²(4,4′-bipy)₄](ClO₄)₄·2CH₃CN·2H₂O}_∞ (2) (where H₂L¹ and H₂L² are the [2 + 2] condensation products of 1,3-diaminopropane with 2,6-diformyl-4-methylphenol and 2,6-diformyl-4-fluorophenol, respectively), have been studied. The interactions of the complexes with calf thymus-DNA were investigated by UV–vis spectroscopy, CD spectroscopy, and gel electrophoresis. The binding constants of 1 and 2 are 7.2 × 10⁴ and 2.1 × 10⁵ M⁻¹, respectively. The complexes exhibit DNA cleavage activity, with the cleavage process involving oxidative cleavage of DNA.

The DNA cleavage activities of two new coordination polymers of Robson-type macrocycles, {[Cu₂L¹(bipy)]·(ClO₄)₂·CH₃CN}_∞ (1) and {[Cu₂L²(bipy)]·(ClO₄)₂·H₂O}_∞ (2), have been studied. The interactions of the complexes with calf thymus-DNA were studied by UV–vis and CD spectroscopic techniques. The complexes exhibit good DNA cleavage activity. The results show that the substituent on the benzene ring plays an important role in DNA cleavage and the cleavage process occurs via oxidative cleavage.

Keywords:

• Dinuclear copper coordination polymers
• DNA cleavage
• Mechanism

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financial support from the National Nature Science Foundation of China [grant number 21301131], [grant number 21477118]; the Nature Science Foundation of Hubei Province [grant number 2013 CFB313]; the open fund of the Engineering Research Center of Nano-Geo Materials of the Ministry of Education China University of Geosciences, Wuhan, the scientific research fund of the Wuhan Institute of Technology [grant number K201471]; the Key Program of the Natural Science Foundation of Hubei Province [grant number 2014CFA530]; the Foundation of the Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology [grant number RGCT200804].