Abstract
Synthesis of [Co(L)(H2O)(HOCN(CH3)2)](ClO4) (where L denotes N,N′-bis(3-methoxysalicylidene)-1,2-diaminopropane) was reported. The complex was characterized by 1H NMR, elemental analysis, IR and UV–vis spectroscopy and the structural features have been solved by X-ray crystallography. The crystal structure analysis showed that the arrangement around cobalt atom is octahedral with two imine nitrogen atoms (N1 and N2) and two oxygen atoms of hydroxyl groups (O2 and O3) at equatorial positions and an aqua ligand and a DMF ligand at the axial positions. The hydrogen-bonding interactions between coordinated H2O and phenoxo/methoxy oxygens connect the complex molecules in dimers. Interaction of the complex with FS-DNA and protein (BSA) was explored by fluorescence and absorption spectroscopy, viscosity measurements, circular dichroism (CD), cyclic voltammetry (CV) and agarose gel electrophoresis. According to the Kb value about 103, the complex is moderately bound to DNA. The complex interacts with protein (BSA) by both static and dynamic quenching. According to cleavage experiment, the complex destroys annular DNA and causes double strand break. Molecular docking was done to achieve detailed binding information of the complex to DNA and BSA. Moreover, cytotoxic activity was evaluated by MTT assay. The complex has low toxicity against the MCF-7 and LNCaP cancer cells.