Early identification of waxflower (Chamelaucium) hybrids using RGAP markers

Figures & data

Fig. 1  Comparison of Chamelaucium uncinatum variety Alba with interspecific hybrid Laura Mae Pearl

(C. uncinatum×C. megalopetalum).

Fig. 2  Hypothesis of hybrid early identification using RGAP markers.

Table 1  NBS-sequence derived primer sequences

Primers	Sequences (5′ → 3′)	Annealing temperature (°C)
P loop-F	GGI GGI GTI GGI AAR ACN AC	53.2
GLPL1	GAG NGC NAG NGG NAG NCC	51.5
Kin2-A	AC ATC GTC IAG AAC TAG IAG	47.2

Fig. 3  Gel image of RGAP DNA markers A and B generated by P loop-F and GLPL1 primers (P1: parent C. uncinatum; P2: parent C. megalopetalum).

Table 2  Biodiversity of waxflower progeny and their parents

Code	Plant number	Flowering time	Bud colour	Petal colour^1	Receptacle colour^1	MFD^2 (mm)	Leaf colour^1	Leaf shape	MLL^3 (mm)
P1	1–5	June to August	Grey-orange 165D	Purple N78C	Purple N79C	16a	Yellow-green 144A	Narrow linear	11.5a
P2	6–8	July to September	Grey-orange 164A	White NN155D change to red-purple 58A as ages	Yellow-green N144C	12.6d	Green 143A	Elliptical	6.8c
H1	9–13	June to August	Grey-orange 164A	White NN155D change to red-purple 67A as ages	Yellow-green 144B	14bc	Green N134A	Narrow linear	10.5b
H2	14–15	Late May to August	Grey-orange 164A	White NN155D with shade of purple N78D	Yellow-green 144B	14.9b	Green N134A	Narrow linear	11.9a
H3	16	May to August	Grey-orange 164A	White NN155D	Yellow-green 144B	15ab	Green N134A	Narrow linear	10.1b
H4	17	May to September	Grey-orange 164A	White NN155D with shade of purple N78D	Yellow-green 144B	16a	Green N134A	Narrow linear	11.5a
RH1	18–20	Late May to September	Grey-orange 164A	White NN155D with shade of purple N78B	Yellow-green 144C	14.8b	Green N134A	Narrow linear	12a
RH2	21	June to September	Grey-orange 164A	White NN155D	Yellow-green 144B	14.8b	Green N134B	Narrow linear	10.4b
RH3	22	July to September	Grey-orange 164A	White NN155C change to being with shade of red-purple 67C	Yellow-green 144B	13.2cd	Green 143A	Narrow linear	10.6b
LSD	(P < 0.05)					1.07			0.84
^1Colour was determined by colour chart of Royal Horticultural Society (5th edition)
^2MFD = mean values of flower diameter within the same column followed by different lower case letters were significantly different at P < 0.05
^3MLL = mean values of leaf length within the same column followed by different lower case letters were significantly different at P < 0.0