Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p

Figures & data

Table 1. LncRNA LINC00324 expression and clinicopathological features.

Subjects	LINC00324 expression		p Values
	Low (n = 59)	High (n = 61)
Age (years)			.664
≤45	39	38
>45	20	23
Tumour size (cm)			.459
≤4	33	30
>4	26	31
Menopause
No	40	34	.174
Yes	19	27
HPV infection
Negative	24	29	.449
Positive	35	32
Lymph node metastasis			.013
Negative	48	37
Positive	11	24
TNM stage			.028
I–II	45	35
III–IV	14	26

Table 2. Multivariate Cox analyses of prognosis of cervical cancer patients.

Subjects	Multivariate Cox analysis
	HR	95% CI	p Value
LINC00324	3.830	1.581–9.278	.003
Age	1.116	0.486–2.564	.796
Tumour size	1.577	0.687–3.624	.283
Menopause	1.404	0.597–3.303	.437
HPV infection	1.192	0.520–2.732	.679
Lymph node metastasis	3.746	1.091–12.867	.036
TNM stage	4.303	1.263–14.664	.020

Figure 1. Expression and relationship of LINC00324 and miR-195-5p in cervical cancer tissue. (A) LINC00324 in cancer tissues was higher than that in normal tissue. (B) RT-qPCR analysis of miR-195-5p was relatively decreased. (C) The expressions of LINC00324 and miR-195-5p were negatively correlated (r = –0.6926, p < .001). (D) LINC00324 was significantly upregulated in cervical cancer cells. (E) MiR-195-5p was downregulated in cells. (F) Transfection efficiency of si-LINC00324 in Hela and SiHa cells. ***p < .001.

Figure 2. LINC00324 interacted with miR-195-5p. (A) LINC00324 had binding sites for miR-195-5p. (B, C) MiR-195-5p mimic weakened the relative luciferase activity of WT-LINC00324 in Hela and SiHa cells. (D, E) The changes in the proliferation ability of the knockdown LINC00324 cells were detected by CCK-8 method. (F, G) Transwell assay examined the influences of knockdown LINC00324 on migration and invasion of Hela and SiHa cells. ***p < .001, ^###p < .001.

Figure 3. Prognostic significance of LINC00324 in cervical cancer prognosis. Kaplan–Meier’s curves for cervical cancer patients with low and high expression of LINC00324 (log-rank p = .032).

Data availability statement

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.