Borax attenuates oxidative stress, inflammation, and apoptosis by modulating Nrf2/ROS balance in acrylamide-induced neurotoxicity in rainbow trout

Abstract

Acrylamide (ACR) can have adverse environmental effects because of its multiple applications. Relevant scientific literatures of the existence of ACR residues in foods following processing steps have raised concern in the biochemistry, chemistry and safety of this vinyl substance. The interest has focused on the hepatotoxicity of ACR in animals and humans and on the ACR content mitigation and its detoxification. Borax (BX), as a naturally occurring antioxidant featured boron compound, was selected in this investigation to assess its possible neuro-protective potential against ACR-induced neurotoxicity. Nrf2 axis signaling pathways and detoxification response to oxidative stress after exposure to ACR in brains of rainbow trout, and the effect of BX application on reducing ACR-induced neurotoxicity were investigated. Rainbow trout were acutely exposed to ACR (12.5 mg/L) alone or simultaneously treated with BX (0.75 mg/L) during 96h. The exposed fish were sampled at 48th and 96th and oxidative stress response endpoints, 8-OHdG, Nrf2, TNF-α, caspase-3, in addition to IL-6 activities and the levels of AChE and BDNF in brain tissues of rainbow trout (Oncorhynchus mykiss) were evaluated. Samples showed decreases in the levels of ACR-mediated biomarkers used to assess neural toxicity (SOD, CAT, GPx, AChE, BDNF, GSH), increased levels of MDA, MPO, DNA damage and apoptosis. ACR disrupted the Nrf2 pathway, and induced neurotoxicity. Inhibited activities’ expressions under simultaneous administration experiments, revealed the protective effects of BX against ACR-induced toxicity damage. The obtained data allow the outline of early multi-parameter signaling pathways in rainbow trout

Keywords:

• Acrylamide
• borax
• oxidative stress
• neuroprotection
• Nrf2
• rainbow trout

Ethics approval

Experiments were performed according to the OECD 210 guideline (OECD, 2013) and approved by Local Ethical Committee for Animal Studies of Ataturk University (Acceptance No:11/2021).

Authors contributions

Gonca Alak: Conceptualization, Formal analysis, Visualization, Writing – Original Draft, Writing – Review & Editing. Arzu Ucar, Veysel Parlak, Aslı Cilingir Yeltekin, Fatma Betul Ozgeris, Hasan Turkez, Gonca Alak, Muhammed Atamanalp: Resources and formal analysis. Muhammed Atamanalp and Nicoleta Anca Şuţan: Writing – Review & Editing, All the authors read and approved the final version.

Disclosure statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Data availability statement

The datasets generated during the current study are available from the corresponding author on request.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.