Liquid–Liquid Extraction of GSH Using DEHAP/Octanol Reverse Micelles

Abstract

In this study, di-(2-ethylhexyl) ammonium phosphate (DEHAP)/octanol reverse micellar extraction was investigated and demonstrated to be an effective method to separate GSH from yeast fermentation broth. The effect of several important factors i.e., pH, DEHAP concentration, and type & concentration of cations on both extraction and strip-extraction was investigated. The optimum operating conditions for both extraction and strip-extraction were obtained by two groups of orthogonal experiments. At the optimum operating conditions, the yield of GSH can reach 68%. In the lyophilized product, the purity of GSH was higher than 75%, all the proteins can be separated, and only a small portion of amino acids were left.

Keywords:

• DEHAP reverse micelles
• GSH
• reverse micellar extraction

ACKNOWLEDGMENTS

The authors would like to thank the Graduate Innovation Fund of Chongqing University and the Chongqing Hanfan Company for their financial support.