One Step Large-Scale Separation and Purification of Rutin from Boenninghausenia sessilicarpa by Countercurrent Chromatography

Abstract

In this study, a preparative countercurrent chromatography (CCC) method for isolation and purification of the bioactive component rutin directly from the ethanol extract of Boenninghausenia sessilicarpa was successfully established by using n-butanol-ethyl acetate-water as the two-phase solvent system. The upper phase of n-butanol-ethyl acetate-water (4:1:5, v/v) was used as the stationary phase of CCC. Under the optimum conditions, 112 mg of rutin at 98.6% purity was obtained from 2.0 g of the crude extract in a single CCC separation. The peak fraction of CCC was identified by negative ESI, ¹H NMR, and ¹³C NMR.

Keywords:

• Boenninghausenia sessilicarpa
• countercurrent chromatography
• preparative chromatography
• rutin

ACKNOWLEDGEMENTS

Y.L. thanks the financial supports from Natural Science Foundation of China (No. 21005072), Department of Science and Technology of Zhejiang Province (No. 2010C12016), Department of Education of Zhejiang Province (No. Y201018030) and Zhejiang Gongshang University through Grant x10-3 and 1110XJ200968.