Optimizing two-phase system by experiment and simulation for high-speed counter-current chromatographic separation of 2-phenylbutyric acid enantiomer

ABSTRACT

High-speed counter-current chromatographic preparative separation of 2-phenylbutyric acid (2-PBA) was successfully proposed. The two-phase solvent system was composed of n-hexane/butyl acetate/aqueous phase (7:3:10, v/v/v). Factors influencing the chiral separation efficiency (e.g. types and concentrations of chiral selector (CS), pH of aqueous phase and temperature) were investigated. A mathematical model was established and verified by conducting experiments. Results showed that the model predictions were in good agreement with the experimental results. Under optimal conditions, 2-PBA was successfully separated. The monomer purity reached 99.5%, and the recovery of enantiomers was 91–93%.

KEYWORDS:

• Chiral separation
• high-speed counter-current chromatography
• hydroxyethy-β-cyclodextrin
• modelling
• 2-phenylbutyric acid

Funding

This work was supported by the National Natural Science Foundation of China (No. 21376071).

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (No. 21376071).