Enzyme-assisted extraction of cordycepin and adenosine from cultured Cordyceps militaris and purification by macroporous resin column chromatography

ABSTRACT

Enzyme-assisted extraction (EAE) coupled with macroporous resin column chromatography (MRCC) was successfully used for the extraction and purification of cordycepin and adenosine from cultured Cordyceps militaris. After optimized by the response surface methodology (RSM), the extraction yields of cordycepin and adenosine were 86.45% and 70.06%, respectively. Subsequently, under the optimal separation conditions on NKA-II resins (loading 1 BV of extraction solution with pH 8.0, and eluting with 4 BVs of 70% ethanol at a flow rate of 3 BV/h), cordycepin (purity of 32.5%), and adenosine (purity of 39.9%) were obtained with an overall recovery rate more than 90%.

Keywords:

• Adenosine
• cordycepin
• Cordyceps militaris
• enzyme-assisted extraction
• macroporous resin column chromatography
• response surface methodology

Funding

The authors are grateful for financial support from Enterprise Commissioned Program of ERA SUN Groups of Anhui (No. W2014JSKF0223) and the National High-Tech Research and Development Program of China (863 Program; No. 2011AA100801-05).

Additional information

Funding

The authors are grateful for financial support from Enterprise Commissioned Program of ERA SUN Groups of Anhui (No. W2014JSKF0223) and the National High-Tech Research and Development Program of China (863 Program; No. 2011AA100801-05).