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ABSTRACT
A simple and efficient method was developed for the preparation of pedunculoside from the dried barks of Ilex rotunda. Pedunculoside was extracted by heat reflux. The optimal conditions were determined by the response surface methodology (40% ethanol concentration, 14 mL/g solvent/material loading level and 90 min extraction time with the dried barks being extracted twice). After extraction and condensing the crude extract, pedunculoside was directly purified through crystallization in water with the addition of ethyl acetate. Pedunculoside (96.9% purity) was obtained with 48.2% recovery after the extraction and purification. Compared with the reported chromatographic methods, this strategy is simple, eco-friendly, and economical. The purified pedunculoside showed an inhibitory effect on pancreatic lipase in vitro (IC50 = 80.8 μg/mL), suggesting its anti-obesity potential.
Declaration of interest
The authors declare no conflicts of interest.
Funding
This study was supported by Guangzhou Quality R&D Center of Traditional Chinese Medicine, State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University (Guangzhou, China).