Increasing the separation of block cryoconcentration through a novel centrifugal filter-based method

ABSTRACT

The objective of this work was to evaluate the filter addition in centrifugal cryoconcentration. The cryoconcentration process was optimized by Response Surface Method using initial concentration (C₀) and centrifugation speed (CS) (independent variables) and efficiency (η), percentage of concentrate (PC) and solute yield (Y) (dependent variables), under radial and axial freezing conditions. The optimal interactions were 5 (wt.%), 3850 rpm and axial condition, with high final solute concentration, and extraordinary separation, with 86%, 63% and 0.87 (kg/kg) for η, PC and Y, respectively. Therefore, the centrifugal filter cryoconcentration allowed the migration of solutes between the crystals, and the filter improved the separation and elution process of the concentrated solution from the ice.

KEYWORDS:

• Cryoconcentration
• filter
• centrifugation
• RSM
• process parameters

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Additional information

Funding

This work was supported by CONICYT–Chile under the Grant Human Capital Formation Advanced Program National Doctoral Fellowship 2016 (Folio No. 21160514, P. Orellana-Palma); the FONDECYT Project No. 11140747 (Dr. Guillermo Petzold); and USDA-NIFA under Grant No. 2015-67017-23074 (Dr. Pawan Takhar);Comisión Nacional de Investigación Científica y Tecnológica [FONDECYT Project No. 11140747 (Dr. Guillermo Petzo,Human Capital Formation Advanced Program National].