Modeling and optimization of bioactive compounds from chickpea seeds (Cicer arietinum L)

ABSTRACT

The aim of study was to model and optimize the extraction of polyphenols and flavonoids with expressed antioxidant activity from chickpea seeds using a central composite design. The optimal extraction conditions were the extraction time of 145.5 min, ethanol concentration of 83.7% and liquid-to-solid ratio of 24.9 (expressed as cm³ per g). The content of total polyphenol and flavonoid was 2.75 g gallic acid equivalent per kilogram of the dried extract and 0.147 g rutin equivalent per kilogram of the dried extract, respectively. Using 2,2-diphenyl-1-picrylhydrazyl assay, it was found that the optimal extract had the IC₅₀ of 1.55 mg cm⁻³.

KEYWORDS:

• Chickpea
• total polyphenol
• total flavonoid
• antioxidant activity
• central composite design

Additional information

Funding

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia under the project TR-34012.