ABSTRACT
This study aimed at extracting and separating anthocyanins from Nitraria tangutorun Bobr. fruit using deep eutectic solvent (DES) combined with macroporous resins. Choline chloride/1,2-propanediol (ChCl-Prop)-based DESs produced higher extraction yields of total anthocyanins from N. tangutorun fruit than pure water and ethanol/methanol/acetone aqueous solutions did, and LX-32 macroporous resin was used to separate total anthocyanins from the DES (recovery>95%). Additionally, a green and efficient HPLC-DAD method was used for the analysis of total anthocyanins using ethanol and a tartaric acid aqueous solution as mobile phases, and 13 anthocyanins were identified from N. tangutorun fruit using a UPLC-Q-TOF-MS method.
Abbreviations: DES, deep eutectic solvent; ChCl-Prop, choline chloride/1,2-propanediol.
Acknowledgements
This work was supported by the Fundamental Research Funds for the Central Universities (GK201603110, GK201706009), the Shaanxi Province Industry-University-Research Cooperative Innovation Project (2017XT-007) and the National Key R&D Program of China (2018YFC1706504). We thank Prof. Zhe-zhi Wang for his generosity in sharing his lab facilities.
Conflict of Interest
All authors declare that they have no conflict of interest to disclose.
Supplementary Material
Supplemental data for this article can be accessed here.