Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection

Figures & data

Table 1. Distribution of mce genes within the order Actinomycetales.

Suborder	Family	Species	mce	Source
Corynebacterineae	Mycobacteria ceae	M. leprae TN	6	UniProt
		M. bovis AF2122/97	18	UniProt
		MTB CDC1551	24	TIGR
		MTB H37Rv	24	TIGR
		Mycobacterium paratuberculosis K-10	48	UniProt
		M. smegmatis MC2 155	34	TIGR
		Mycobacterium sp. MCS	38	JGI
		Mycobacterium sp. KMS	38	JGI
		Mycobacterium sp. JLS	50	JGI
		Mycobacterium flavescens PYR-GCK	48	UniProt
		Mycobacterium vanbaalenii PYR-1	66	UniProt
	Nocardiaceae	N. farcinica IFM 10152	36	UniProt
Micrococcineae	Intrasporangiaceae	Janibacter sp. HTCC2649	6	NCBI
Propionibacterineae	Nocardioidaceae	Nocardioides sp. JS614	12	UniProt
Pseudonocardineae	Pseudonocardiaceae	Amycolatopsis mediterranei	6	Pfam
Streptomycineae	Streptomycetaceae	Streptomyces avermitilis MA-4680	6	UniProt
		S. coelicolor A3(2)	6	UniProt

Table 2. List of MAP cell surface proteins and their functions.

S. No.	Mycobacterium structural proteins	Functions
1	MAP2189	Mammalian cell entry proteins
2	MAP2190
3	MAP2191
4	MAP2192
5	MAP2193
6	MAP2194
7	MAP3567	Hypothetical protein
8	MAP1508	Hypothetical protein
9	MAP 0047c	Lpp-LpqN family conserved in Mycobacteria ceae
10	MAP0209c	Protein potentially involved in peptidoglycan biosynthesis in MAP
11	MAP3936	Chaperonin GroEL
12	MAP4143	Elongation factor Tu
13	MAP3024c	HupB
14	MAP3651c	FadE3_2
15	MAP1997	Acyl carrier protein
16	MAP3968	Heparin-binding hemagglutinin adhesin-like protein
17	MAP1122	MIHF
18	MAP1589c	Alkylhydroperoxidase C
19	MAP1506	Hypothetical protein
20	MAP3362c	S-adenosyl-L-homocysteine hydrolase
21	MAP1519	Hypothetical protein
22	MAP2698c	DesA2 DesA2
23	MAP1998	3-oxoacyl-(acyl carrier protein) synthase II
24	MAP3840	Molecular chaperone DnaK
25	MAP4264	co-chaperonin GroES
26	MAP3693	Acetyl-CoA acetyltransferase
27	MAP1563c	Hypothetical protein
28	MAP0398c	Probable transcriptional regulatory protein
29	MAP0896	Succinyl-CoA synthetase subunit beta
30	MAP0966c	Hypothetical protein
31	MAP3033c	SerA
32	MAP3007	Hypothetical protein
33	MAP3188	FadE24
34	MAP0990	Phosphopyruvate hydratase
35	MAP1588c	AhpD
36	MAP1164	Glyceraldehyde-3-phosphate dehydrogenase
37	MAP1889c	Wag31
38	MAP4233	DNA-directed RNA polymerase subunit alpha
39	MAP4167	rpsC
40	MAP3061c	Probable electron transfer flavoprotein (beta-subunit) fixed
41	MAP2228	Hypothetical protein
42	MAP4233	DNA-directed RNA polymerase subunit alpha
43	MAP2453c	AtpH
44	MAP3005c	Hypothetical protein
45	MAP2280c	ATP-dependent Clp protease proteolytic subunit

Figure 1. Schematic diagram of M. tuberculosis mce operons. Transcription regulators are colored in brown, yrbE genes in blue, mce genes in green, mas genes in yellow and genes encoding Mce-family lipoprotein (lpr) are shown in purple.

Table 3. Classification of MAP and MTB H37Rv yrbE and mce genes.

Prefix^a	yrbE1A	yrbE1B	mce1A	mce1B	mce1C	mce1D	mce1E	mce1F
MAP	3602	3603	3604	3605	3606	3607	3608	3609
MTB	0167	0168	0169	0170	0171	0172	0173	0174
	yrbE2A	yrbE2B	mce2A	mce2B	mce2C	mce2D	mce2E	mce2F
MAP	4082	4083	4084	4085	4086	4087	4088	4089
MTB	0587	0588	0589	0590	0591	0592	0593	0594
	yrbE3A	yrbE3B	mce3A	mce3B	mce3C	mce3D	mce3E	mce3F
MAP	2117^b	2117^b.1^c	2116^b	2115^b	2114^b	2113^b	2112^b	2111^b
MTB	1964	1965	1966	1967	1968	1969	1970	1971
	yrbE4A	yrbE4B	mce4A	mce4B	mce4C	mce4D	mce4E	mce4F
MAP	0562	0563	0564	0565	0566	0567	0568	0569
MTB	3451^b	3450^b	3499^b	3498^b	3497^b	3496^b	3495^b	3494^b
	yrbE5A	yrbE5B	mce5A	mce5B	mce5C	mce5D	mce5E	mce5F
MAP	–	–	2189	2190	2191	2192	2193	2194
MTB	–	–	–	–	–	–	–	–
	yrbE6A	yrbE6B	mce6A	mce6B	mce6C	mce6D	mce6E	mce6F
MAP	–	–	–	–	–	–	–	–
MTB	–	–	–	–	–	–	–	–
	yrbE7A	yrbE7B	mce7A	mce7B	mce7C	mce7D	mce7E	mce7F
MAP	1849	1850	1851	1852	1853	1854	1855	1856
MTB	–	–	–	–	–	–	–	–
	yrbE8A	yrbE8B	mce8A	mce8B	mce8C	mce8D	mce8E	mce8F
MAP	–	–	–	–	–	–	–	–
MTB	–	–	–	–	–	–	–	–

^aOrganism specific gene number prefix: MAP; MTB H37Rv.

^bOrthologous sequence present, but Open Reading Frame (ORF) annotated in reverse direction.

^cOrthologous sequence present, but not annotated. ORF extends ∼400 bp at 5′end.