Figures & data
Figure 1. Generation and characterization of VSV/RABV-GP. A: VSV/RABV-GP production was measured by sandwich ELISA. Results have been expressed as the mean of OD450 ± SD values and are representative of at least three independent experiments. Dotted line indicates the background. B: BHK21 cells were transduced with VSV/RABV-GP or VSV/VSV-GP in the presence or absence of the indicated antibodies. Virus entry was quantified by measuring the luciferase levels at 18-h post-transduction. Error bars represent the SD from the mean (n = 3). Statistical significance was assessed by Student’s t test. *p < 0.05, ***p < 0.001; ns, not significant.
Figure 2. Immunization schedule and humoral immune response induced by vaccination. A: Schematic diagram of the immunization protocol. BALB/c mice were i.p. immunized with PBS, RABA, or VSV/RABV-GP at 0 and 14 days after the first immunization. At 28 days post-immunization, mice were sacrificed and serum samples were collected. B and C: Detection of the levels of RABV GP specific IgM (B) and IgG (C). Results are expressed as the mean (n = 6) of OD450 ± SD values and are representative of at least three independent experiments. Statistical significance was assessed by Student’s t test. ***p < 0.001; ns, not significant.
Figure 3. Influence of VSV/RABV-GP immunization on the type of T helper cell response. The serum specific IgG1 (A) and IgG2a (B) titers were determined as indicators of a Th2 or Th2 type of response, respectively. C: IgG1/IgG2a titer ratios of immunized mice. Results are expressed as the mean (n = 6) of OD450 ± SD values and are representative of at least three independent experiments. Statistical significance was assessed by Student’s t test. ns, not significant.
Figure 4. Protective efficacy of VSV/RAVB-GP vaccination. Detection of neutralizing antibody titers in the serum of the immunized mice by fluorescent antibody virus neutralization test (FAVNT). Results are expressed as the mean (n = 3) of serum neutralizing titers ± SD values and are representative of at least three independent experiments. Statistical significance was assessed by Student’s t test. *p < 0.05.
