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Abstract
There is little information regarding the chromosomal regions conferring zinc (Zn) accumulation in barley. With the aim of developing markers for Zn accumulation, 150 lines derived from cross between ‘Clipper’ (low-Zn-accumulator) and ‘Sahara’ (high-Zn-accumulator) were screened. In field-grown plants, two regions located on 2HS and 2HL were associated with seed Zn concentration and content. 2HS was flanked by Xbcd175 and Xpsr108; 2HL was flanked by vrs1 and XksuF15 markers. These two regions accounted for 45% of total variation in seed Zn concentration and 59% of total variation in seed Zn content. In a glasshouse experiment, 2HS and 2HL were also associated with seed Zn concentration and content, and explained 37% and 55% of the total variation in seed Zn concentration and content, respectively. The identification of these Quantitative Trait Loci (QTL) provides an important starting point for transferring and pyramiding genes that may contribute to the improvement of barley productivity and nutritional quality in Zn-deficient environments.