Effect of Ozone on Viability of Activated Sludge Detected by Oxygen Uptake Rate (OUR) and Adenosine-5′-triphosphate (ATP) Measurement

Abstract

The current study focused on treatment of phenolic wastewater using an integrated process – dosing of ozone directly to activated sludge. The main goal was to analyze the effect of ozonation on viability of activated sludge in different systems – activated sludge in distilled water and activated sludge in wastewater. Two viability detection methods, oxygen uptake (OUR) rate and adenosine-5'-triphosphate measurement (ATP), were compared. The linear correlation between ATP and OUR measurements in studied range was found to be good (r² = 0.90). In case of ozonation of activated sludge in wastewater, ozone doses up to 42 mgO₃·gMLVSS⁻¹ did not influence the viability of sludge. In addition, contrary to ozonation of sludge in distilled water, soluble COD was reduced by 15.6% (at ozone dose of 42 mgO₃·gMLVSS⁻¹).

Keywords:

• Ozone
• Phenolic Wastewater
• Activated Sludge
• Viability
• Adenosine-5′-triphosphate
• Oxygen Uptake Rate
• Oil Shale

ACKNOWLEDGMENTS

This research was supported by the Estonian targeted funding projects 0690063s08 and 0142719s06.