Resonance Light Scattering Method for Study on the Interaction Between Fluorinated Surfactant Potassium Perfluorooctanesulfonate and DNA

Abstract

Resonance light-scattering (RLS) characteristics of the interactions between fluorinated surfactant potassium perfluorooctanesulfonate (PFOS) with calf thymus nucleic acid (ct DNA) were studied. After DNA was added, aggregation of PFOS on the molecular surface of DNA occurred in the pH 3.5 ∼6.0, which resulted in an enhanced RLS peak at 370 nm. The RLS intensity was found to be proportional to the concentration of DNA. The determination limit was 20.0 μg · L⁻¹. UV-visible (UV-vis) spectra and infrared (IR) spectra both proved the binding changed the confromation of DNA.

Keywords:

• Aggregation
• DNA
• fluorinated surfactant
• resonance light-scattering

The Natural Science Foundation of Hubei Province of China and Ministry of Education, Key Laboratory for the Synthesis and Application of Organic Functional Molecules supported this work; all the authors here express their deep thanks.

Notes

0.002 mg · L⁻¹ Al³⁺; 0.03 mg · L⁻¹K⁺; 0.05 mg · L⁻¹ RNA; 0.06 mg · L⁻¹ Br⁻; 0.02 mg · L⁻¹ Zn²⁺; 0.08 mg · L⁻¹; 0.16 mg · L⁻¹ Ba²⁺; Ni²⁺; 0.36 mg · L⁻¹ Ca²⁺; pH = 5.5.