Figures & data
Figure 1. Fundamental of RNA interference.
Figure 2. Pictorial representation of naked siRNA structure with its important feature of 7 bp seed region, its cleavage site at position between 10 and 11 plus its two double overhang nucleotide at 3′ end. The passenger strand and the guide strand are represented in blue and orange, respectively.
Figure 3. A schematic representation of an optimized siRNA molecule with the phosphorothioate (P=S) and 2′-base sugar modifications (green circles) with exonuclease and endonuclease abilities, respectively.
Figure 4. A schematic illustration of construction of enzymatically synthesized siRNA library.
Figure 5. Schematic representation of 4 different siRNA mutation targeting strategies as: (a) Direct Targeting- siRNA design specific to mutant one (b) Indirect targeting – siRNA design as SNP variant of mutant transcript (c) Exon specific – siRNA targeting to only specific exon as blue one shown here (d) Exon skipping – siRNA design to skip exon but boundaries of exon are targeted.
Table 1. Current status of siRNA drug development programs.
Figure 6. Schematic representation of conventional and modified siRNA-based MBs.
