European proficiency testing of national reference laboratories for the confirmation of sulfonamide residues in muscle and milk

Abstract

Two interlaboratory studies were organized in 2002–2003 in order to check the proficiency of laboratories in confirming the presence of sulfonamide residues in muscle and milk. These studies involved 25 EU National Reference Laboratories (NRLs) from 21 different European countries in charge of statutory monitoring of antimicrobial residues in food of animal origin at a national level. The study was conducted according to international and national guidelines by the Community Reference Laboratory (CRL) in charge of antimicrobial substances. Four different test matrices of sheep muscle and four different test matrices of bovine milk containing different sulfonamide substances were prepared and sent to the participants. Each participant was asked to use his own routine confirmatory method and to analyse each sample in triplicate within a period of about six weeks during which the stability of the materials was checked by the organizer. The sulfonamide content of each material was determined by calculating the robust means of all the results and the deviation of the results from the assigned values was assessed by calculating Z-scores. Overall, results were satisfactory, particularly considering that it was the first proficiency test dealing with sulfonamides organised by the Community Reference Laboratory.

Keywords:

• Proficiency testing
• sulfonamides
• animal tissue
• milk
• veterinary drug residues

Acknowledgements

Many thanks to Stéphane Marteau and Jean-Guy Rolland, the technical staff of the experimental farm, for their help in the preparation of test materials.