Comparison of a fluoroquinolone surface plasmon resonance biosensor screening assay with established methods

Abstract

The performance of a previously developed immunochemical biosensor screening method for fluoroquinolone (FQ) antibiotics in poultry muscle, fish and egg was compared with established methods. Blank sample material of the target matrices was individually spiked with the FQs at half maximum residue levels. Homogeneity of the test materials was confirmed by liquid chromatography–mass spectrometry (LC–MS/MS). Identical sets of spiked samples as well as incurred samples from a previous feeding experiment were sent to three independent laboratories and analysed by LC–MS/MS, a microbiological assay and the new biosensor assay. The new method correctly identified all contaminated samples and demonstrated advantages in sensitivity and analysis time compared to the microbiological screening assay.

Keywords:

• immunoassay
• surface plasmon resonance
• microbiological assay
• LC–MS/MS
• veterinary drug residues
• poultry
• fish
• egg

Acknowledgements

This project was financially supported by the European Commission, project “New technologies to screen multiple chemical contaminants in foods”, contract FOOD-CT-2004-06988, acronym BioCop.