Figures & data
Figure 1. Anti-tumor effects of combination therapies using LH and cytokine cocktails in B16-BL6-bearing mice. (a) Growth curves of B16 tumors on the foot-pad in each group. The 4 treatment groups are described in the text. The average tumor size on day 25 was lower in the LH plus 12 cytokines group than in the LH or PBS groups (P < 0.05 by two-tailed t-test). (b) Survival curve, P < 0.05 for LH plus 12 cytokines vs. PBS or 12 cytokines (log rank test).
Figure 2. Anti-tumor effects of combination therapies using local hyperthermia (LH) and cytokine cocktails in Lewis lung carcinoma (LLC)-bearing mice. (a) Growth curves of the LLC tumors on the foot-pad in each group. The 6 treatment groups are described in the text. The average tumor size on day 21 was lower in the LH plus Th1 cytokines and LH plus 12 cytokines groups than in the control group (P < 0.01 by two-tailed t-test). (b) Survival curve. P < 0.05 in LH plus Th1 cytokines vs. PBS, and in LH plus 12 cytokines vs. 12 cytokines, P < 0.01 in LH plus 12 cytokines vs. PBS or LH (log rank test). The LH plus IFNs group was excluded for the reason described in the text.
Figure 3. (a)–(h) Hematoxylin and eosin histochemical staining. Spleen (a, b), Lymph nodes (c), and thymus (d) in a B16 mouse cured by the LH plus 12-cytokine treatment or those (e, f, g, h) from the LH group, respectively. (i, j) Immunohistological staining with anti-CD3 rat monoclonal antibody of spleen in the LH plus 12-cytokine group (i) and one in the LH group (j). (original magnification, a, c–j, 400×, b, f, 1000×).
Figure 4. (a)–(f) Hematoxylin and eosin histochemical staining. (a, b) Lung of a B16 mouse cured by the LH plus 12-cytokine treatment. (c) Lung from the LH group. B16 metastasis with melanin pigment is shown. Liver (d), kidney (e) and small intestine (f) from the LH plus 12-cytokine group. (g) Immunohistological staining with anti-CD3 monoclonal antibody. Lung of a B16 mouse cured by the LH plus 12-cytokine treatment. (original magnification a, c, d, e, f, g, 400×, b, 1000×).
