Figures & data
Figure 1. Experimental schedule: (a) first series of experiments; (b) second series of experiments; (c) third series of experiments.
Table I. Criteria for scoring gross morphologic damage of the colo Citation[14], Citation[16].
Figure 2. The kinetics of a rectal temperature curve during immersion in a water bath at (a) 42°C and (b) 36.5°C.
Figure 3. Macroscopic findings of colonic mucosa in TNBS-induced rat colitis. Rats were given a single enema of TNBS (30 mg in 50% ethanol/rat) and were treated in a water bath maintained at 42°C for 20 min (Hyperthermia; HT) after TNBS administration as described in detail in ‘Materials and methods’. (a) The macroscopic changes of the luminal side of the distal colon were observed 6 days after the enema of TNBS. (b) Effect of HT on the damage score 6 days after the enema of TNBS. *Values are the means ±SEM of 6–8 mice. p < 0.01 vs. sham, #p < 0.05 vs. TNBS enema.
Figure 4. Histological changes, tissue MPO activities in the colon after TNBS treatment. (a) Microscopic findings of colonic mucosa in TNBS-induced rat colitis. (b) Effect of HT on the increase of neutrophil accumulation in colonic mucosa 6 days after the enema of TNBS. Values are the means ±SEM of 6–8 mice. *p < 0.001 vs. sham, #p < 0.05 vs. TNBS enema.
Figure 5. Effect of hyperthermia on colonic expression of tumor necrosis factor-α. Values are the means ±SEM of 6–8 mice. *p < 0.05 vs. sham.
Figure 6. Induction of heat shock proteins in rat colon. (a) Hsp32 and Hsp70 inductions after HT. (b) Hsp32 and Hsp70 inductions after the enema of TNBS. (c) Enhanced effect of HT on the TNBS-induced Hsp32 and Hsp70.
Figure 7. Effect of zinc protoporphyrin η on hyperthermia in TNBS-induced colitis. (a) Effects of zinc protoporphyrin IX on the damage score in TNBS colitis treated with HT. *p < 0.01 vs. sham, #p < 0.05 vs. TNBS. (b) Effects of zinc protoporphyrin IX on colonic CINC-1 levels in TNBS colitis treated with HT. Values are the means ± SEM of 6–8 mice.
Figure 8. Effect of geranylgeranylacetone on hyperthermia in TNBS-induced colitis. (a) Effects of GGA on HT-induced Hsp70 in colonic mucosa. (b) The macroscopic changes of the luminal side of the distal colon were observed 6 days after the enema of TNBS. (c) Effects of GGA on the damage score in TNBS colitis treated with HT. *p < 0.01 vs. sham, #p < 0.05 vs. TNBS, ##p < 0.01 vs. TNBS.
