Changes in peripheral lymphocyte subsets in patients after partial microwave ablation of the spleen for secondary splenomegaly and hypersplenism: A preliminary study

Figures & data

Table I.  Changes in T lymphocytes after microwave ablation therapy (mean ± SD).

Phenotype	CD3^+ (%)	CD4^+ (%)	CD8^+ (%)	CD4^+/CD8^+
Pre-therapy	64.0 ± 15.32	32.5 ± 10.36	32.1 ± 13.30	1.28 ± 0.90
Post-therapy
1 day	60.7 ± 14.17	29.9 ± 8.90	29.1 ± 15.59	1.39 ± 0.95
3 days	62.0 ± 13.60	30.3 ± 10.89	30.2 ± 14.79	1.34 ± 0.93
7 days	64.10 ± 12.47	31.8 ± 8.52	31.2 ± 11.44	1.18 ± 0.57
1 month	70.63 ± 7.05*	38.5 ± 9.10*	31.88 ± 8.08	1.30 ± 0.47
3 months	66.29 ± 13.95	33.86 ± 10.38	30.14 ± 17.56	1.37 ± 0.63
F	2.58	2.84	0.28	0.21
P	0.04	0.028	0.92	0.95

F and P were the value comparison between pre- and post-therapy. *Significantly different from pre-therapy level, p < 0.05.

Table II.  Changes in peripheral blood B lymphocytes and NK cells after microwave ablation therapy (mean ± SD).

Phenotype	B lymphocytes (%)	NK cells (%)
Pre-therapy	12.1 ± 5.93	9.7 ± 6.73
Post-therapy
1 day	15.3 ± 8.42	10.3 ± 4.72
3 days	16.4 ± 9.79	9.3 ± 3.59
7 days	15.9 ± 8.43	7.4 ± 2.76
1 month	14.3 ± 3.95	10.4 ± 4.24
3 months	15.8 ± 4.88	8.8 ± 3.87
F	6.47	1.39
P	0.0002	0.25

F and P were the value comparison between pre- and post-therapy.

Table III.  Changes in T lymphocytes subsets grouped by the ablation volume (mean ± SD).

	CD3^+ (%)		CD4^+ (%)		CD8^+ (%)		CD4^+/CD8^+
	AV ≤ 20%	AV 20–40%	AV ≤ 20%	AV 20–40%	AV ≤ 20%	AV 20–40%	AV ≤ 20%	AV 20–40%
Phenotype	(group A)	(group B)	(group A)	(group B)	(group A)	(group B)	(group A)	(group B)
Pre-therapy	61.2 ± 13.4	66.8 ± 18.1	30.6 ± 13.6	34.4 ± 6.9	32.2 ± 15.3	32.0 ± 12.8	1.30 ± 1.20	1.25 ± 0.60
Post-therapy
1 day	58.0 ± 11.1	63.4 ± 17.6	27.8 ± 8.3	32.0 ± 9.9	28.0 ± 13.8	30.2 ± 18.8	1.19 ± 0.62	1.60 ± 1.23
3 days	60.6 ± 11.8	63.4 ± 16.5	30.6 ± 12.8	30.0 ± 10.1	26.6 ± 15.2	33.8 ± 15.1	1.56 ± 1.13	1.12 ± 0.73
7 days	60.0 ± 13.1	68.2 ± 11.6	30.0 ± 11.1	33.6 ± 5.73	28.0 ± 14.5	34.4 ± 7.6	1.35 ± 0.76	1.01 ± 0.26
1 month	71.0 ± 7.40	70.0 ± 7.94	37.4 ± 11.8	40.3 ± 0.57	33.2 ± 8.6	29.7 ± 8.3	1.22 ± 0.54	1.43 ± 0.38
3 months	62 ± 16.95	72.0 ± 8.19	27.0 ± 8.04	43.0 ± 2.65*	33.3 ± 23.4	26.0 ± 7.8	1.08 ± 0.60	1.75 ± 0.50

*Significantly different from the ablation volume ≤ 20% group at the same occasion, p < 0.05.

Table IV.  Changes in B lymphocytes and NK cells groups by ablation volume (mean ± SD).

	B lymphocytes (%)		NK cell (%)
	AV ≤ 20%	AV 20–40%	AV ≤ 20%	AV 20–40%
Phenotype	(group A)	(group B)	(group A)	(group B)
Pre-therapy	9.4 ± 1.81	14.8 ± 7.59	11.8 ± 8.70	7.6 ± 3.91
Post-therapy
1 day	12.6 ± 2.41	18.0 ± 11.6	11.6 ± 3.71	9.0 ± 5.65
3 days	12.4 ± 3.05	20.4 ± 12.89	10.2 ± 2.04	8.4 ± 4.77
7 days	12.6 ± 4.61	19.2 ± 10.54	8.8 ± 1.48	6.0 ± 3.16
1 month	13.0 ± 1.15	16.0 ± 6.08	11.6 ± 5.03	8.3 ± 1.53
3 months	13.5 ± 1.29	19.5 ± 9.19	8.75 ± 4.35	9.0 ± 4.24
F	3.25	3.53	0.60	1.11
P	0.029	0.024	0.70	0.39

F and P were the value comparison between pre- and post-therapy.