Figures & data
Figure 1. Changes in expression of NQO1 in tumor cells after heating at 42°C for 1 h. (A) Western blots of NQO1 in FSaII fibrosarcoma cells of C3H mice and A549 human lung cancer cells. (B) Relative changes in NQO1 levels in FSaII cells and A549 cells, as determined from the density of the Western blots. Averages of 4 determinations with 1 SD are shown. (C) Immunofluorescent staining for NQO1 in FSaII cells and A549 cells at different times after heating at 42°C for 1 h.
Figure 2. Survival curves of FSaII cells and A549 cells treated with 2.5–10.0 µM β-lap for 4 h. Averages of 6 experiments with duplicate cultures and 1 SE are shown.
Figure 3. Changes in β-lap sensitivity after heating. Clonogenic survivals of FSaII cells (A) and A549 cells (B) were determined after the cells were heated at 42°C for 1 h and then treated with 5 µM β-lap for 4 h at various times after heating. The effects of heating alone or β-lap treatment alone are also shown. The dotted lines indicate the expected percentage cell survival if the combined effect of heating and β-lap treatment was additive. Averages of 6–7 experiments with duplicate cultures and 1 SE are shown.
Figure 4. Effect of pre-heating at different temperatures on the clonogenic survival of FSaII cells and A549 cells to β-lap treatment applied 24 h after heating. Black columns: cells were treated with 5 µM β-lap for 4 h at 37°C. Gray columns: cells were heated at different temperatures for 1 h. Open columns: cells were heated at different temperatures for 1 h, and then treated 24 h later with 5 µM β-lap for 4 h. Averages of 5–7 experiments with duplicate cultures and 1 SE are shown.
Figure 5. Growth of FSaII tumors in the hind legs of C3H mice after receiving various treatments. Heat alone: tumors were heated for 1 h with 42°C water-bath. β-lap alone: host mice were treated with an i.p. injection of 50 mg/kg β-lap. Heat, 24 h, β-lap: tumors were heated, and then the host mice were treated 24 h later with β-lap. Averages of 7–8 tumors and 1 SE are shown.
