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Current Eye Research Volume 33, 2008 - Issue 5-6
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Original Article

Reliability of Different Image Analysis Methods for Scanning Laser Doppler Flowmetry

Andreas J. Kreis Centre for Eye Research Australia, University of Melbourne, Australia
,
Thanh Nguyen Centre for Eye Research Australia, University of Melbourne, Australia
,
Sophie Rogers Centre for Eye Research Australia, University of Melbourne, Australia
,
Jie Jin Wang Centre for Eye Research Australia, University of Melbourne, Australia; Centre for Vision Research, Westmead Millennium Institute, University of Sydney, Australia
,
Joanna Harazny University Eye Hospital of Erlangen, University of Erlangen, Germany
,
Georg Michelson University Eye Hospital of Erlangen, University of Erlangen, Germany
,
H. M. Omar Farouque Department of Cardiology, Austin Health Hospital, University of Melbourne, Australia
&
Tien Y. Wong Centre for Eye Research Australia, University of Melbourne, Australia; Singapore Eye Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
 show all
Pages 493-499 | Received 19 Dec 2007, Accepted 14 Mar 2008, Published online: 02 Jul 2009
 
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Abstract

Purpose: To assess reliability and reproducibility of different analysis methods for retinal capillary flow, volume, and velocity from scanning laser Doppler flowmetry (SLDF) topography. Material and Methods: SLDF topography analysis using the default retinal flowmeter (HRF) were compared to that using automatic full-field perfusion image analyzer (AFFPIA) and quantified as intraclass correlation coefficients (ICC). Results: The AFFPIA full-field method had the highest reliability, with ICC 0.99 for capillary flow. The reproducibility using the AFFPIA full-field method was high ICC 0.74 for capillary flow. Conclusions: The AFFPIA full-field method is highly reliable and superior to the default HRF software.

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