Effects of cadmium on metallothionein and histology in gills of tilapia (Oreochromis niloticus) at different salinities

ABSTRACT

The objectives of this study were to evaluate the effects of sublethal cadmium concentrations on the levels of cadmium, metallothionein (MT) and histological changes in gills of East Java strain tilapia (Oreochromis niloticus) at different salinity levels. The levels of cadmium in control gills were not significantly different at 0, 5 and 10 practical salinity unit (PSU). The cadmium concentrations in gills of cadmium-exposed fish were significantly higher at 0 PSU than at 5, 10 and 15 PSU. The MT concentrations in control gills were not significantly different at 0, 5, and 10 PSU. The MT concentrations of cadmium-exposed fish were significantly higher than those in respective control groups at 0, 5 and 10 PSU. Significant gill damage occurred in fish exposed to cadmium at lower salinity. The epithelial lifting was noted at gills of fish exposed to 2.5 mg/L of cadmium at 0 PSU, and telangiectasis was observed at gills exposed to 5 mg/L of cadmium at 0 PSU. The level of gill damage decreased with increasing salinity of media. The increased MT and histological changes in gills of our findings could be a protective response of animals to toxic effect of cadmium.

KEYWORDS:

• Cadmium
• accumulation
• metallothionein
• gills
• histology
• Oreochromis niloticus

Acknowledgments

The authors would like to thank Dr. N. Usman and Mr. Setiyanto for technical assistance during experiment.

Disclosure statement

The authors declare that there are no conflicts of interest.

Additional information

Funding

This study was supported by research mandate of Airlangga University and Kementerian Riset, Teknologi dan Pendidikan Tinggi Indonesia (Indonesian Ministry of Research, Technology and Higher Education).