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Abstract
Interest in biomass biorefineries and especially their aldonic acid products has been growing in recent years. However, the analytical methods for aldonic acids have only rarely been explored. In this study, a simple one-step method was developed using high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). With this method, five wood monosaccharides and their corresponding aldonic acids, including arabinose, galactose, glucose, xylose, mannose, arabonic acid, galactonic acid, gluconic acid, xylonic acid, and mannonic acid, were simultaneously separated and quantitatively determined. The separation was performed on a CarboPacTM PA-10 column (250 mm × 2 mm) with a gradient elution using NaOH solution as the mobile phase. The calibration curves showed good linearity (R2 ≥ 0.9993) for the five monosaccharides and the corresponding aldonic acids in the range of 0.1 to 10.0 mg/L. The spiked recoveries of the 10 components ranged from 93.92% to 104.15%, with relative standard deviations (RSDs) (n = 3) of 0.49% to 3.95%. The established method was applied successfully to determine the quantitative variation of monosaccharide and aldonic acid contents in products from a biomass biorefinery.
Acknowledgments
This work was supported by a grant from the National Natural Science Foundation of China (No. 31370573) and a grant from the National High Technology Research and Development Program of China (No. 2012AA022304). The authors were also grateful for the financial support from Jiangsu Province Science and Technology Supported Project (BE2011838) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).