Lignin and Other Aromatic Substances Released from Spruce Wood During Pressurized Hot-Water Extraction, Part 1: Extraction, Fractionation and Physico-Chemical Characterization

Abstract

Ground spruce wood was extracted with pressurized hot water in an accelerated solvent extractor (ASE) at 170°C during 20, 60, and 100 min. Released aromatic substances (from 2.2 to 2.5% on wood basis) were isolated on XAD-7 resin and fractionated into lignin, oligomeric aromatic substances (OAS), and compounds insoluble in methanol (IMC). The separated aromatic fractions, and the ground wood before and after extraction, were characterized by physico-chemical methods (GC, GC-MS, HPLC-SEC, TG, DSC). The major part of the aromatic substances was oligomeric aromatic substances (OAS). This fraction increased significantly with the extraction time, while the yield of dissolved lignin increased only slightly with extraction time. Isolated lignins had a lower molar mass and differed considerably from spruce milled wood lignin (MWL). The isolated lignins were more stable against oxidative thermodegradation than spruce MWL. The UV-extinction coefficients at 280 nm were lower for the isolated lignins than for MWL.

KEYWORDS:

• Hot-water extraction
• lignin characterization
• lignin isolation
• spruce wood
• XAD-7 resin