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Original Articles

Assessment of Bioaerosol Generation and Sampling Efficiency Based on Pantoea agglomerans

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Pages 620-628 | Received 16 Jun 2008, Accepted 09 Feb 2009, Published online: 24 Mar 2009

Figures & data

FIG. 1 Laboratory setup of the generation-to-sampling train; APS = aerodynamic particle sizer; T = temperature; RH = relative humidity.

FIG. 1 Laboratory setup of the generation-to-sampling train; APS = aerodynamic particle sizer; T = temperature; RH = relative humidity.

FIG. 2 Top and side views of the bubble nebulizer.

FIG. 2 Top and side views of the bubble nebulizer.

FIG. 3 Correlation of APS counts with optical (hemacytometer) counts. PSL beads of 0.8 μ m, 1.07 μ m, 2.1 μ m, 2.9 μ m, and 4.5 μ m were counted.

FIG. 3 Correlation of APS counts with optical (hemacytometer) counts. PSL beads of 0.8 μ m, 1.07 μ m, 2.1 μ m, 2.9 μ m, and 4.5 μ m were counted.

FIG. 4 Typical distribution of P. agglomerans inside the chamber, measured with the APS.

FIG. 4 Typical distribution of P. agglomerans inside the chamber, measured with the APS.

FIG. 5 Culturability of the nebulizer fluid before and after nebulization, plotted by nebulizer. N = 28. The box extends from the 25th to the 75th percentile. The line across the box represents the median value. The whiskers extend to the most extreme observations that are within 1.5 times the percentiles. Points outside this range represent outliers. * denotes statistical significance.

FIG. 5 Culturability of the nebulizer fluid before and after nebulization, plotted by nebulizer. N = 28. The box extends from the 25th to the 75th percentile. The line across the box represents the median value. The whiskers extend to the most extreme observations that are within 1.5 times the percentiles. Points outside this range represent outliers. * denotes statistical significance.

FIG. 6 Culturability efficiency (CE). Pooled data (n = 29) from aerosolized bacteria sampled with BioSampler and AGI-30, plotted by generator (Log scale).

FIG. 6 Culturability efficiency (CE). Pooled data (n = 29) from aerosolized bacteria sampled with BioSampler and AGI-30, plotted by generator (Log scale).

FIG. 7 Culturability efficiency (CE) for the BioSampler and AGI-30 impinger (n = 16). Data obtained using the bubble nebulizer and plotted by sampler (Log scale).

FIG. 7 Culturability efficiency (CE) for the BioSampler and AGI-30 impinger (n = 16). Data obtained using the bubble nebulizer and plotted by sampler (Log scale).

FIG. 8 Culturability efficiency (CE) data (n = 26 for water; n = 47 for PBS) plotted by collection media.

FIG. 8 Culturability efficiency (CE) data (n = 26 for water; n = 47 for PBS) plotted by collection media.

FIG. 9 Culturability efficiency as a function of relative humidity; (a) values at RH < 20% and (b) values at RH > 20%.

FIG. 9 Culturability efficiency as a function of relative humidity; (a) values at RH < 20% and (b) values at RH > 20%.

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