Inactivation of Airborne Influenza Virus by Tea Tree and Eucalyptus Oils

Figures & data

FIG. 1 Laboratory setup for experiments with oils coated filters.

FIG. 2 ESEM image of uncoated fiber.

FIG. 3 Laboratory setup for aerosolized oils experiments.

FIG. 4 ESEM image of TTO-coated fiber.

FIG. 5 Particle size distributions of aerosolized oils and virus suspension. Error bars represent standard deviation of at least three experimental runs.

FIG. 6 Inactivation of influenza virus by oils in liquid medium. Error bars represent standard deviation of at least three experimental runs.

TABLE 1 Reference fluorescence of the material loaded onto the filters. The data cover all experiments with filters

	Relative fluorescence (RFU)
Exposure time (min)	Mean values	Std. Dev.
0	55,010	7059
15	57,283	6337
30	53,824	6428
45	57,279	5947

TABLE 2 Relative survival of influenza A virus on the filter surface, treated with oils

	Relative survival (%)
Exposure time (min)	LMO	EUO	TTO
0	87.03	1.26	0
15	75.58	0	0
30	66.85	0	0
45	55.45	0	0

TABLE 3 Inactivation of airborne influenza virus by aerosolized TTO

	Viral concentration (PFU/mL)		Relative fluorescence (RFU)
Exposure time (min)	w/o oil	TTO	w/o oil	TTO
	Averages and standard deviations of four experimental repeats
5	150 ± 45.46	5 ± 1.82	26,900 ± 2332	20,360 ± 1997
15	60 ± 18.25	ND	21,100 ± 1980	19,830 ± 1985
30	40 ± 8.16	ND	18,220 ± 1865	18,760 ± 1735
60	20 ± 8.16	ND	14,620 ± 1267	14,520 ± 1389
ND – not determined.

FIG. 7 Inactivation of influenza virus by oils on the filter surface. Error bars represent standard deviation of at least three experimental runs.