Glucocorticoid receptor subunit gene expression in thyroid gland and adenomas

Figures & data

Table I.  The sequences of the primer and Taqman probe.

GR-α amplification primer and fluorescent probe sequences

Forward primer 5′-TGAAAATGGGTTGGTGCTTCTA-3′

Reverse primer 5′-GACAAGAATACTGGAGATTTGAGTCAA-3′

Fluorescentprobe 5′-FAM-CCTGATGGCACTTAGCTATCAGAAGACCACAA-TAMRA-3′

GR-β amplification primer and fluorescent probe sequences

Forward primer 5′-TGGCCACCCCAAAAGGA-3′

Reverse primer 5′-GAGCTCATCCCATGCTAATTATCC-3′

Fluorescentprobe

5′-FAM- AACTAACATGATTTGTGTCTATGAAGTGC-TAMRA-3′

Figure 1.  Fluorescent intensity curves for GR–a standard, from left to right the concentration of standard is 1×10⁸, 1×10⁷, 1×10⁶ and 1×10⁵ copies/ml.

Figure 2.  Fluorescent intensity curves for GR–β standard, from left to right the concentration of standard is 1×10⁸, 1×10⁷, 1×10⁶ and 1×10⁵ copies/ml.

Figure 3.  Fluorescent intensity curves for negative control (distilled water) standard.

Figure 4.  The standard curve for GR–α.

Figure 5.  The standard curve for GR–β.