May the APC gene somatic mutations in tumor tissues influence the clinical features of Chinese sporadic colorectal cancers?

Figures & data

Table I.  Somatic APC mutations detected in tumors of sporadic CRCs.

Nr	Exon	Nucleotide change	Target sequence of mutation	Consequence
CRC2	15	c.4348C > T	CGA→TGA	p.Arg1450X
CRC4	3	c.421_422delAG*	AGAGAGGT→AGAGGT	Framshift
	9	c.994C > T	CGA→TGA	p.Arg332X
	15	c.2626C > T	CGA→TGA	p.Arg876X
	15	c.4663insA	AAAAAA→AAAAAAA	Framshift
CRC5	15	c.2560A > T*	AGA→TGA	p.Arg854X
CRC7	15	c.4270_4360del 91bp*	CTTCCA…AAAAAT→CCT…AAAAT	Frameshift
CRC9	9	c.994 C > T	CGA→TGA	p.Arg332X
	15	c.4663insA	AAAAAA→AAAAAAA	Framshift
CRC13	15	c.4446_4455del 10bp*	TCTTCC…GCTGAT→TCTGAT	Frameshift
CRC15	15	c.4393_4394delAG	AGAGAGAGAGTG→AGAGAGAGTG	Frameshift
CRC16	9	c.1143insG*	CGGGCCAGGGCC→CGGGCGCAGGGCC	Frameshift
	15	c.3340C > T	CGA→TGA	p.Arg1114X
CRC18	15	c.2626C > T	CGA→TGA	p.Arg876X
	15	c.3340C > T	CGA→TGA	p.Arg1114X
	15	c.3566C > A*	TCA→TAA	p.Ser1189X
CRC26	15	c.2872A > T*	AGA→TGA	p.Arg958X
CRC29	15	c.2872A > T*	AGA→TGA	p.Arg958X
CRC31	15	c.4132C > T	CAG→TAG	p.Gln1378X
CRC32	15	c.3340C > T	CGA→TGA	p.Arg1114X
	15	c.4348 C > T	CGA→TGA	p.Arg1450X
CRC33	3	c.421_422delAG*	AGAGAGGT→AGAGGT	Framshift
	15	c.2926insA	GGTAAAAGA→GGTAAAAAGA	Frameshift
	15	c.4393_4394delAG	AGAGAGAGAGTG→AGAGAGAGTG	Frameshift
CRC37	15	c.3340C > T	CGA→TGA	p.Arg1114X
CRC38	15	c.4234delG*	AGGGAATG→AGGAATG	Frameshift
CRC40	15	c.2555T > A*	TTG→TAG	p.Leu852X
	15	c.4348 C > T	CGA→TGA	p.Arg1450X
CRC43	15	c.3340C > T	CGA→TGA	p.Arg1114X

*novel mutations.

Table II.  Correlation between APC mutations and the age of diagnosis of the CRC.

Age of diagnosis (yrs)	With 1 mutation	With ≥ 2 mutations	Without mutation	Ratio of mutation
∼49	3	6	4	69.2%
50∼69	7	1	15	34.7%
≥70	1	0	6	14.2%
	Wilcoxon rank test: p = 0.048
	Pearson correlation Coefficients: r = − 0.382, p = 0.012

Table III.  Correlation between APC mutations and the age/pathological features of tumors.

	With biallelic mutations	With allelic mutations	Without mutation	p-value*
Age of diagnosis
< 50 (yrs) (n = 13)	4	5	4	p = 0.004
≥ 50 (yrs) (n = 30)	0	9	21
Tumor localization
Rectum (n = 27)	2	7	18	p = 0.54
Colon (n = 16)	2	7	7
Grading
II (n = 35)	3	12	20	p = 1.000
III (n = 8)	1	2	5
Dukes’ stage
A/B (n = 28)	1	7	20	p = 0.03
C/D (n = 15)	3	7	5
Pattern of cancer growth
Expanding (n = 17)	0	2	15	p = 0.003
Infiltrating (n = 26)	4	12	10

*Fisher's exact test.

Table IV.  Correlation between mutation at codon1114 and the metastasis status of the tumors.

	With metastasis	Without metastasis
With mutation at codon1114	5	0
With mutations at another loci	5	8
p-value*	0.03

*Fisher's exact test.

Figure 1.  Hot spots of APC somatic mutations in western and Asian sporadic CRC patients.