L1CAM expression in colorectal cancer identifies a high-risk group of patients with dismal prognosis already in early-stage disease

Figures & data

Figure 1. Intense cytoplasmic staining of neoplastic and membranous cells for L1CAM (A) (X100). (B) (X200). (C) Absence of L1CAM staining in normal colonic epithelium. There is L1CAM staining in the ganglionic cells (x200).

Table 1. Correlation of L1CAM expression with clinicopathological characteristics.

Colorectal cancer N = 109	L1CAM				p
	No or low expression		High expression
	N	% within L1CAM	N	% within L1CAM
Stage
I + II	46	64.8	8	21.1	<.001
III + IV	25	35.2	30	78.9
T
1	12	16.9	2	5.3	(T1-3 vs T4) .040
2	19	26.8	4	10.5
3	34	47.9	23	60.5
4	6	8.5	9	23.7
N
0	49	69.0	9	23.7	<.001
1 + 2	22	31.0	29	76.3
M
no	65	91.5	27	71.1	.011
yes	6	8.5	11	28.9
G
1 + 2	54	76.1	27	71.1	.647
3	17	23.9	11	28.9
V
No	55	77.5	26	68.4	.360
Yes	16	22.5	12	31.6
Pn
No	67	94.4	33	86.8	.272
Yes	4	5.6	5	13.2
Mucinous
No	66	93.0	34	89.5	.717
Yes	5	7.0	4	10.5
Position
Right sided	21	29.6	10	26.3	.937
Left sided	23	32.4	13	34.2
Rectum	27	38.0	15	39.5

Figure 2. Kaplan–Meier curve depicting progression-free survival (A) and overall survival (B) in months of the study population according to the levels of expression of L1CAM (low vs high). Kaplan–Meier curve illustrating progression-free survival (C) and overall survival (D) of patients with early stage cancer only (stage I and II) and low versus high expression of L1CAM.

Table 2. Multivariate analysis regarding progression free survival (PFS) and overall survival (OS) using cox proportional hazard models.

Multivariate analysis for PFS	Sig.	Exp(B)	95.0% CI for Exp(B)
			Lower	Upper
T	0.035	0.166	0.032	0.878
N	0.013	0.341	0.147	0.794
M	0.008	0.293	0.118	0.730
G	0.281	2.146	0.535	8.605
V	0.759	0.860	0.328	2.256
Pn	0.607	1.389	0.397	4.861
L1CAM	<0.0001	0.187	0.075	0.467
Multivariate analysis for OS
T	0.058	0.097	0.009	1.087
N	0.010	0.259	0.093	0.720
M	0.004	0.228	0.083	0.622
G	0.970	1.027	0.263	4.003
V	0.073	0.392	0.141	1.090
Pn	0.734	1.254	0.340	4.631
L1CAM	0.001	0.154	0.049	0.483