TMEM27 expression and clinical characteristics and survival in clear cell renal cell carcinoma

Figures & data

Figure 1. TMEM27 immunohistochemistry staining intensities starting from weak to strong membranous positivity (A-F). (A) shows the weakest staining (0.5) while (F) has the strongest membrane staining (3). Each image is at 40X. Scale bar represents 50 µM.

Figure 2. (A) Kaplan–Meier analysis demonstrating the association of TMEM27 IHC staining intensity with overall survival of 321 patients from time of nephrectomy. No stain is tumor that has 0 stain for TMEM27. Positive stain includes tumors with staining intensity from 0.5 to 3.0. The shaded areas around the lines represent the 95% confidence band. (B) Kaplan–Meier analysis demonstrating the association of TMEM27 IHC with disease-free survival in patients without metastasis at time of nephrectomy (N = 285). No stain is tumor that has 0 stain for TMEM27. Positive stain includes tumors with staining intensity from 0.5 to 3.0. The shaded areas around the lines represent the 95% confidence band.

Table 1. Patient demographics based on intensity of TMEM27 immunohistochemistry staining.

TMEM27 Immunohistochemistry Staining Intensity	[0](No Stain)	[0.5 – 3](Positive Stain)	[0 – 3](All)
Cases (n)	153	168	321
Age (years) (mean ± standard deviation)	60.5 ± 12.3	61.4 ± 12.2	61.0 ± 12.2
Male (%)	56.9	63.1	60.1
Tumor size (cm) (mean ± standard deviation)	6.2 ± 3.6	4.7 ± 2.8	5.4 ± 3.3
Cases with metastasis at time of nephrectomy	25	11	36
Cases with metastasis at latest follow up	44	26	70
Deaths	48	34	82

Table 2. Correlation between TMEM27 staining and clinical characteristics.

Clinical Characteristic	r value	p-value
Tumor size	−0.191	<0.001
TNM stage (pT)	−0.145	<0.01
TNM stage (pN)	−0.152	NS
Furhman grade	−0.187	<0.001
Sarcomatoid rhabdoid present	−0.073	NS
Large vessel venous invasion	−0.113	<0.05
Lymphatic invasion	−0.072	NS
Metastasis at time of nephrectomy	−0.126	<0.05
Metastasis at follow-up	−0.094	NS
Composite	−0.201	<0.001
Composite with Death	−0.202	<0.001
Modified SSIGN Score	−0.218	<0.001

Data availability statement

The data that support the findings of this study are available from the corresponding author, [RG], upon reasonable request.