The BioLymph study – implementing precision medicine approaches in lymphoma diagnostics, treatment and follow-up: feasibility and first results

Figures & data

Figure 1. Overview of collection of materials (biological sampling and questionnaires) in the BioLymph study.

CSF: cerebrospinal fluid; QOL: quality of life; cfDNA: cell-free DNA, PET/CT: positron-emission tomography/computed tomography

Figure 2. Overview of collection of biological materials in the BioLymph study by lymphoma subtype and treatment protocol.

CSF: cerebrospinal fluid; QOL: quality of life; cfDNA: cell-free DNA; PET/CT: positron-emission tomography/computed tomography; RT: radiotherapy

Table 1. Overview of patient characteristics and materials collected at diagnosis, and targeted sequencing performed for the first 100 study participants in the BioLymph study.

Characteristics		Number (%) of patients
Sex	Men Women	62 (62%) 38 (38%)
Age, median (range)		69 (19-94) years
Lymphoma subtype
	Diffuse large B-cell lymphoma^a Follicular lymphoma Hodgkin lymphoma Indolent B-cell lymphomas (excl FL) T-cell lymphomas Mantle cell lymphoma Other/unspecified	33 (33%) 19 (19%) 18 (18%) 16 (16%) 6 (6%) 6 (6%) 2 (2%)
Treatment/management strategy at diagnosis
	Chemo(immuno)therapy Immunotherapy only (anti-CD20 antibody) Radiotherapy only Watch and wait	70 (70%) 3 (3%) 11 (11%) 16 (16%)
Materials
	DNA prepared from diagnostic tumor tissue^b	69 (84%)
	Plasma (EDTA and STRECK tubes) at diagnosis	96 (96%)
	Whole blood for normal DNA	97 (97%)
	Questionnaires completed	92 (92%)
Targeted sequencing panel used^c
	TruSight (fresh frozen tissue)	33 (20)
	GMS Lymphoid	30
	TruSight and GMS Lymphoid	6
Sequencing results
	Number of clinically relevant variants identified per patient^d (median, range)	1 (0–5)

^aincluding transformed follicular lymphoma.

^bexcludes Hodgkin lymphoma cases where special techniques for DNA extraction of tumor cells will be needed.

^c2 samples failed sequencing likely due to low DNA quality.

^dclinically relevant variants were variants classified as class 4 or 5 according to ACMG guidelines.

Data availability statement

Study data are available upon request if in line with ethical and legal permissions.