Using total plasma triacylglycerol to assess hepatic de novo lipogenesis as an alternative to VLDL triacylglycerol

Figures & data

Table 1. Subject characteristics.

Characteristics	Mean ± SD
Age, years	46 ± 6
Sex, M/F	46/17
BMI	29.3 ± 3.9
Glucose, mmol/L	5.5 ± 0.9
Insulin, mU/L	13.5 ± 8.5
Triglycerides, mmol/L	1.5 ± 0.9
Total cholesterol, mmol/L	5.1 ± 1.0
HDL cholesterol, mmol/L	1.2 ± 0.4

Data (mean ± SD) are based on baseline/first visit data (n = 63); follow-up/second visit data were similar (data not shown).

Figure 1. Histograms showing the distributions, means, and medians of DNL_VLDL-TAG and DNL_Plasma-TAG. n = 123.

Figure 2. Correlation between DNL_VLDL-TAG and DNL_Plasma-TAG. n = 123.

Figure 3. Bland–Altman plot showing the agreement between DNL_VLDL-TAG and DNL_Plasma-TAG. n = 123.

Figure 4. Alluvial plot with subjects split into tertiles based on DNL_VLDL-TAG and DNL_Plasma-TAG, showing misclassification of subjects when using DNL_Plasma-TAG. Median (IQR) DNL_VLDL-TAG is shown beside respective tertile. Coloured dots represent composition of the tertile; e.g. red dots (subjects) in the top tertile of DNL_Plasma-TAG actually belong to the middle tertile when using DNL_VLDL-TAG.

Figure 5. Repeatability of DNL measured in VLDL-TAG and Plasma-TAG. (A) Bland-Altman plot showing mean and difference of the two measurements of DNL in VLDL-TAG; (B) Bland-Altman plot showing mean and difference of the two measurements of DNL in Plasma-TAG; (C) Matched-pair plot showing the individual differences between first and second measurement of DNL in VLDL-TAG; (D) Matched-pair plot showing the individual differences between first and second measurement of DNL in Plasma-TAG. n = 16.