Abstract
The analytical method reported herein relates to the detection of glucose in the range of 0.04–5.2 mM, with the aid of in situ-generated carbon nanoparticles (NPs) as a sensor. Hydrophilic carbon NPs with specific absorbance value at 275 nm were obtained in a reaction between glucose and urea at 120ºC for 20 minutes in an autoclave. The afore-mentioned chemical reaction was developed into a chemical sensing process for glucose detection. A good linearity over a wide range of glucose concentration (0.04–5.2 mM) with a correlation coefficient of 0.9948 and a detection limit of 0.04 mM is observed. The current assay for glucose sensing based on UV light absorbing carbon nanoparticles is simple, reproducible and fast. The process is devoid of either electrochemical – enzymatic (glucose oxidase) or non-enzymatic assays reported thus far.