Protection from infectious bursal disease virus (IBDV)-induced immunosuppression by immunization with a fowlpox recombinant containing IBDV-VP2

Figures & data

Fig. 1 Experimental design and time-line showing experimental procedures as a function of days post-hatch. Boxes under the time-line describe the sampling periods for the different serological analyses. Details of the treatment groups and number of animals used for histology and serology are provided. Y, yes; N, No.

Fig. 2 Bursal lesion scores for animals following infection with IBDV F52/70. Ten vaccinated birds were sampled 5 days after infection, and 15 infected birds between 3 and 5 days post-infection, on reaching defined clinical end-points. Birds were vaccinated with fpIBD1 at 7 and 21 days of age, and infected at 31 days of age.

Fig. 3 Antibody responses to IBDV F52/70 infection. Serum antibody was measured by IgM-specific or IgG-specific enzyme-linked immunosorbent assay for IBDV as A₄₉₂±standard error of the mean. Birds were vaccinated at 7 and 21 days of age, and infected at 31 days of age. Details of the treatment groups are given in Fig. 1.

IBDV-specific IgM and IgG response to inactivated vaccine (Nobilis TRT+IBm+G+ND) given 95 days post-hatch

	IgM			IgG
Group	0 days post-vaccination	7 days post-vaccination	18 days post-vaccination	0 days post-vaccination	7 days post-vaccination	18 days post-vaccination
1	0.060±0.017^a	0.228±0.038	0.074 ±± 0.024	0.279±0.044	0.259±0.040	0.252±0.043
2	0.022±0.019	0.114±0.025	0.048±0.022	0.245±0.030	0.249±0.016	0.230±0.043
3	0.090±0.021	0.283±0.052	0.241±0.071	0.270±0.038	0.295±0.041	0.366±0.071
4	0.124±0.019	0.245±0.034	0.239±0.065	0.323±0.053	0.275±0.035	0.431±0.068
^aMean A 492±standard error of the mean. Details of the treatment groups are given in Fig. 1.

Fig. 4 IgM and IgG responses to S. enteritidis bacterin, given 14 days after infection with IBDV F52/70. Values are expressed as A₄₉₂±standard error of the mean. Details of the treatment groups are given in Fig. 1.

IgG and IgM response to revaccination with S. enteritidis vaccine, given 63 days post infection with IBDV

	IgM			IgG
Group	0 days post-vaccination	7 days post-vaccination	18 days post-vaccination	0 days post-vaccination	7 days post-vaccination	18 days post-vaccination
1	0.241±0.026^a	1.504±0.115	1.341±0.143	0.664±0.031	0.907±0.030	1.055±0.022
2	0.408±0.083	1.900±0.154	0.912±0.211	0.509±0.064	0.923±0.057	0.903±0.089
3	0.337±0.075	1.490±0.149	1.059±0.159	0.671±0.044	0.966±0.044	1.041±0.020
4	0.238±0.032	1.615±0.127	1.207±0.168	0.587±0.071	1.041±0.033	1.109±0.018
^aMean A 492±standard error of the mean. Details of the treatment groups are given in Fig. 1.